Vicente J S, Llobat M D, Jiménez-Trigos E, Lavara R, Marco-Jiménez F
Institute of Science and Animal Technology (ICTA), Universidad Politécnica de Valencia, Valencia, Spain.
Reprod Domest Anim. 2013 Jun;48(3):402-6. doi: 10.1111/rda.12087. Epub 2012 Oct 4.
The aim of this work was to study the influence of embryonic and maternal genotype of two lines of rabbits selected by growth rate (line R) and litter size at weaning (line A) on prenatal survival. Embryos were recovered at 48 h of gestation from R and A donors (39 and 35 does, respectively) and reciprocally transferred to the oviducts of recipient does to the R (n = 15) and A (n = 14) lines. Each recipient doe received six embryos from line R into one oviduct and six embryos from line A into the other. Recipient does were examined by laparoscopy to determine implantation rate on day 14 and slaughtered on day 25 of gestation to determine the number of live foetuses and the weight of foetuses and placentas. No differences were found between lines in fertilization rate and stage of embryo development at 48 h post-insemination. Implantation rate was affected by both the embryonic and maternal genotype. While embryos from donor line A had the highest implantation rate (0.78 ± 0.032 vs 0.65 ± 0.036 for line R), recipient line R had a better implantation rate (0.78 ± 0.033 vs 0.64 ± 0.036 for line A). Foetal survival was affected by the embryonic genotype. Embryos from donor line A had a higher foetal survival rate than embryos from donor line R (0.65 ± 0.036 vs 0.53 ± 0.038, respectively) but lower foetal and placenta weights. In conclusion, while embryonic genotype influenced both implantation and foetal survival rate, R embryos had the lowest rates, maternal genotype affected the implantation rate and R recipients may show a greater uterine receptivity during implantation period. Moreover, it must be observed that foetal and placenta weights were significantly affected by embryonic genotype and heavier for R line.
本研究旨在探讨生长速度（R系）和断奶时窝产仔数（A系）选育的两个家兔品系的胚胎基因型和母体基因型对产前存活的影响。在妊娠48小时从R系和A系供体母兔（分别为39只和35只）回收胚胎，并相互移植到R系（n = 15）和A系（n = 14）受体母兔的输卵管中。每只受体母兔在一个输卵管中接受6个R系胚胎，在另一个输卵管中接受6个A系胚胎。在妊娠第14天通过腹腔镜检查受体母兔以确定着床率，并在妊娠第25天屠宰以确定活胎数量以及胎儿和胎盘的重量。授精后48小时，两品系在受精率和胚胎发育阶段方面未发现差异。着床率受胚胎基因型和母体基因型两者的影响。虽然来自供体A系的胚胎着床率最高（R系为0.65±0.036，A系为0.78±0.032），但受体R系的着床率更好（A系为0.64±0.036，R系为0.78±0.033）。胎儿存活率受胚胎基因型影响。来自供体A系的胚胎比来自供体R系的胚胎有更高的胎儿存活率（分别为0.65±0.036和0.53±0.038），但胎儿和胎盘重量较低。总之，胚胎基因型影响着床率和胎儿存活率，R系胚胎的这两项比率最低，母体基因型影响着床率，且R系受体在着床期可能表现出更高的子宫接受性。此外，必须注意到胎儿和胎盘重量受胚胎基因型显著影响，且R系更重。
