用白细胞介素6 cDNA转染的白细胞介素6依赖性B细胞的生长自主性和致瘤性。
Growth autonomy and tumorigenicity of interleukin 6-dependent B cells transfected with interleukin 6 cDNA.
作者信息
Tohyama N, Karasuyama H, Tada T
机构信息
Department of Immunology, Faculty of Medicine, University of Tokyo, Japan.
出版信息
J Exp Med. 1990 Feb 1;171(2):389-400. doi: 10.1084/jem.171.2.389.
Abstract
We introduced an IL-6 cDNA expression vector into a murine B cell line, the growth of which definitely required the presence of exogenous IL-6. The transfected cells secreted substantial amounts of IL-6, to which they themselves responded by proliferating without further requirement of exogenous IL-6. The proliferation was a direct function of cell density and was inhibitable by antibodies to IL-6, indicating the autocrine nature of the growth. The IL-6 cDNA-transfected cells displayed greatly enhanced tumorigenicity when inoculated into syngeneic and nude mice. Our data suggest that an IL-6 autocrine self stimulation confers on B cells a selective growth advantage and results in the induction of progression of the malignant state of B cells.
摘要
我们将白细胞介素-6（IL-6）的互补DNA（cDNA）表达载体导入一种小鼠B细胞系，该细胞系的生长绝对需要外源性IL-6的存在。转染后的细胞分泌大量IL-6，它们自身对其作出反应，通过增殖而不再需要外源性IL-6。这种增殖是细胞密度的直接函数，并且可被抗IL-6抗体抑制，表明这种生长具有自分泌性质。当将IL-6 cDNA转染的细胞接种到同基因和裸鼠体内时，它们表现出大大增强的致瘤性。我们的数据表明，IL-6自分泌自我刺激赋予B细胞选择性生长优势，并导致B细胞恶性状态进展的诱导。
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本文引用的文献
1
Induction of plasma-cell neoplasms in strain BALB/c mice with mineral oil and mineral oil adjuvants.用矿物油和矿物油佐剂在BALB/c品系小鼠中诱导浆细胞瘤。
Nature. 1962 Mar 17;193:1086-7. doi: 10.1038/1931086a0.
2
Autocrine secretion and malignant transformation of cells.细胞的自分泌分泌与恶性转化。
N Engl J Med. 1980 Oct 9;303(15):878-80. doi: 10.1056/NEJM198010093031511.
3
Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor.人类皮肤T细胞淋巴瘤和白血病细胞系可产生并对T细胞生长因子作出反应。
J Exp Med. 1981 Nov 1;154(5):1403-18. doi: 10.1084/jem.154.5.1403.
4
Transforming growth factor(s) production enables cells to grow in the absence of serum: an autocrine system.转化生长因子的产生使细胞能够在无血清条件下生长：一种自分泌系统。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):485-9. doi: 10.1073/pnas.79.2.485.
5
Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor.猿猴肉瘤病毒癌基因v-sis源自编码血小板衍生生长因子的基因。
Science. 1983 Jul 15;221(4607):275-7. doi: 10.1126/science.6304883.
6
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Proc Natl Acad Sci U S A. 1980 Apr;77(4):2163-7. doi: 10.1073/pnas.77.4.2163.
7
The synergism of accessory cells and of soluble alpha-factors derived from them in the activation of B cells to proliferation.辅助细胞及其衍生的可溶性α因子在激活B细胞增殖过程中的协同作用。
Immunol Rev. 1984 Apr;78:51-74. doi: 10.1111/j.1600-065x.1984.tb00476.x.
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9
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