Department of Microbiology, School of Biotechnology, Faculty of Pharmacy, University of Santiago de Compostela, 15782, Spain.
J Agric Food Chem. 2012 Oct 31;60(43):10796-807. doi: 10.1021/jf3027974. Epub 2012 Oct 18.
Foam production is an essential characteristic of beer, generated mainly from the proteins present in the malt and, to a minor extent, from the mannoproteins in brewer's yeast cell walls. Here, we describe the isolation and characterization of the novel fermentation gene CFG1 (Carlsbergensis foaming gene) from Saccharomyces pastorianus. CFG1 encodes the cell wall protein Cfg1p, a 105 kDa protein highly homologous to Saccharomyces cerevisiae cell wall mannoproteins, particularly those involved in foam formation, such as Awa1p and Fpg1p. Further characterization of Cfg1p revealed that this novel protein is responsible for beer foam stabilization. This report represents the first time that a brewing yeast foaming gene has been cloned and its action fully characterized.
泡沫产生是啤酒的一个重要特征，主要由麦芽中存在的蛋白质产生，在较小程度上也由啤酒酵母细胞壁中的甘露糖蛋白产生。在这里，我们描述了从酿酒酵母中分离和鉴定新型发酵基因 CFG1（嘉士伯起泡基因）的过程。CFG1 编码细胞壁蛋白 Cfg1p，这是一种 105 kDa 的蛋白质，与酿酒酵母细胞壁甘露糖蛋白高度同源，特别是那些与泡沫形成有关的蛋白，如 Awa1p 和 Fpg1p。对 Cfg1p 的进一步表征表明，这种新型蛋白质负责啤酒泡沫的稳定。本报告首次克隆了酿造酵母起泡基因并对其作用进行了全面表征。
