通过多重 PCR 鉴定经济上重要的美洲斑潜蝇属物种（双翅目：潜蝇科）的引物设计。

Primer design for identifying economically important Liriomyza species (Diptera: Agromyzidae) by multiplex PCR.

机构信息

Miyagi Prefectural Institute of Agriculture and Horticulture, Takadate-kawakami, Natori, Miyagi 981-1243, Japan.

出版信息

Mol Ecol Resour. 2013 Jan;13(1):96-102. doi: 10.1111/1755-0998.12025. Epub 2012 Oct 8.

DOI:10.1111/1755-0998.12025

Abstract

Leafminer flies, especially, Liriomyza huidobrensis, Liriomyza sativae and Liriomyza trifolii, are quarantine species in many countries. Their morphological similarity makes identification difficult. To develop a rapid, reliable, sensitive and simple molecular identification method using multiplex PCR, we newly sequenced the mitochondrial cytochrome oxidase I (COI) genes of Liriomyza bryoniae, Liriomyza chinensis, L. huidobrensis, L. sativae, L. trifolii, Chromatomyia horticola and four parasitoid species. We aligned them with all the COI sequences of the leafminer flies found in the international DNA nucleotide sequence databases (DDBJ/EMBL/GenBank). We then designed species-specific primers to allow us to differentiate between L. bryoniae, L. chinensis, L. huidobrensis, L. sativae, and L. trifolii.

摘要

潜叶蝇属的蝇类，尤其是南美斑潜蝇、美洲斑潜蝇和三叶草斑潜蝇，在许多国家都是检疫性物种。它们的形态相似，使得鉴定变得困难。为了开发一种快速、可靠、敏感和简单的基于多重 PCR 的分子鉴定方法，我们新测序了美洲斑潜蝇、南美斑潜蝇、美洲斑潜蝇、美洲斑潜蝇、三叶斑潜蝇、番茄斑潜蝇和四种寄生性天敌的线粒体细胞色素氧化酶 I（COI）基因。我们将它们与在国际 DNA 核苷酸序列数据库（DDBJ/EMBL/GenBank）中发现的所有潜叶蝇属蝇的 COI 序列进行了比对。然后，我们设计了种特异性引物，以区分 L. bryoniae、L. chinensis、L. huidobrensis、L. sativae 和 L. trifolii。