Bayliss J M, Reeves W B, Andreoli T E
Department of Internal Medicine, University of Arkansas College of Medicine, Little Rock 72205.
J Membr Biol. 1990 Jan;113(1):49-56. doi: 10.1007/BF01869605.
This paper provides the results of studies which characterized conductive 36Cl- flux in basolaterally enriched membrane vesicles prepared from rabbit renal outer medulla. Conductive 36Cl- uptake was studied under two different experimental conditions. In the first, 36Cl- flux was driven by an inside positive voltage created with oppositely directed Cl- and gluconate gradients. In the second, an inwardly direct K+ gradient was used to drive 36Cl- uptake. By these two methods, voltage-sensitive 36Cl- uptake was shown to comprise about 45 and 65%, respectively, of the initial rates of total 36Cl- flux. Separate paired studies demonstrated that the conductive 36Cl- uptake was inhibited by the Cl- channel blocker diphenylamine-2-carboxylate (DPC) with an IC50 for DPC of 154 microM. The voltage-dependent 36Cl- uptake had an activation energy of 6.4 kcal/mole. This 36Cl- conductance had an anion selectivity sequence of I- greater than Cl- greater than or equal to NO3- much greater than gluconate.
本文提供了一些研究结果，这些研究对从兔肾外髓制备的基底外侧富集膜囊泡中的氯离子（(^{36}Cl^-)）传导通量进行了表征。在两种不同的实验条件下研究了氯离子（(^{36}Cl^-)）的传导性摄取。第一种情况下，(^{36}Cl^-)通量由相反方向的氯离子（(Cl^-)）和葡萄糖酸盐梯度产生的内向正电压驱动。第二种情况下，向内的钾离子（(K^+)）梯度用于驱动(^{36}Cl^-)摄取。通过这两种方法，电压敏感的(^{36}Cl^-)摄取分别占总(^{36}Cl^-)通量初始速率的约45%和65%。单独的配对研究表明，氯离子通道阻滞剂二苯胺 - 2 - 羧酸盐（DPC）可抑制传导性(^{36}Cl^-)摄取，DPC的半数抑制浓度（IC50）为154微摩尔。电压依赖性(^{36}Cl^-)摄取的活化能为6.4千卡/摩尔。这种(^{36}Cl^-)传导性的阴离子选择性顺序为：碘离子（(I^-)）＞氯离子（(Cl^-)）≥硝酸根离子（(NO_3^-)）＞＞葡萄糖酸盐。
