Southwestern University, 1001 E. University Avenue, Georgetown, TX 78626, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Nov 1;908:155-60. doi: 10.1016/j.jchromb.2012.09.001. Epub 2012 Sep 7.
The identification and quantitation of carisoprodol (Soma) and its chief metabolite meprobamate, which is also a clinically prescribed drug, remains a challenge for forensic toxicology laboratories. Carisoprodol and meprobamate are notable for their widespread use as muscle relaxants and their frequent identification in the blood of impaired drivers. Routine screening is possible in both an acidic/neutral pH screen and a traditional basic screen. An improvement in directed testing quantitations was desirable over the current options of an underivatized acidic/neutral extraction or a basic screen, neither of which used ideal internal standards. A new method was developed that utilized a simple protein precipitation, deuterated internal standards and a short 2-min isocratic liquid chromatography separation, followed by multiple reaction monitoring with tandem mass spectrometry. The linear quantitative range for carisoprodol was determined to be 1-35mg/L and for meprobamate was 0.5-50mg/L. The method was validated for specificity and selectivity, matrix effects, and accuracy and precision.
卡利卓多（Soma）及其主要代谢产物美普他酚的鉴定和定量分析对法医毒理学实验室来说仍然是一项挑战。卡利卓多和美普他酚被广泛用作肌肉松弛剂，经常在受损驾驶员的血液中被发现。酸性/中性 pH 筛选和传统碱性筛选都可以进行常规筛选。与当前的非衍生酸性/中性提取或碱性筛选选项相比，需要改进有针对性的测试定量方法，因为这两种方法都没有使用理想的内标。开发了一种新方法，该方法利用简单的蛋白质沉淀、氘代内标和 2 分钟的等度液相色谱分离，然后进行串联质谱的多重反应监测。卡利卓多的线性定量范围为 1-35mg/L，美普他酚的线性定量范围为 0.5-50mg/L。该方法经过了特异性和选择性、基质效应以及准确性和精密度的验证。
