通过聚合酶合成硒代亚甲基锁定核酸（SeLNA）修饰的寡核苷酸。
Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases.
机构信息
School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane 4072, Australia.
出版信息
Chem Commun (Camb). 2012 Nov 18;48(89):11020-2. doi: 10.1039/c2cc36464f.
PMID:23042489
Abstract
Enzymatic recognition of SeLNA nucleotides was investigated. KOD XL DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of SeLNA-modified DNA templates was also efficiently achieved by Phusion and KOD XL DNA polymerases.
摘要
研究了 SeLNA 核苷酸的酶识别。发现 KOD XL DNA 聚合酶在引物延伸反应中是一种有效的酶。Phusion 和 KOD XL DNA 聚合酶也有效地实现了 SeLNA 修饰的 DNA 模板的聚合酶链反应 (PCR) 扩增。
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