Janulíková J, Staneková Z, Mucha V, Kostolanský F, Varečková E
Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9,845 05 Bratislava, Slovak Republic.
Acta Virol. 2012;56(3):169-76. doi: 10.4149/av_2012_03_169.
Currently, a new trend in development of vaccines against influenza with broader spectrum of efficacy is focused on conserved antigens of influenza virus. The HA2 glycopolypeptide (HA2 gp) is one of conserved antigens, potentially suitable as immunogens inducing cross-protection against influenza. We selected two distinct domains of HA2 gp originating from influenza A virus (IAV) of H3 subtype for induction of antiviral immune response: the ectodomain (EHA2) comprising aa 23-185 and the fusion peptide (FP) comprising N-terminal aa 1-38. BALB/c mice were immunized with three doses of EHA2 and FP, respectively, and subsequently challenged with 2 LD50 of IAV of homologous (H3) or heterologous (H7) HA subtype. Both peptides induced significant antibody response and protected mice against the lethal infection. The most efficient protection was achieved with EHA2 against homologous virus.
influenza A virus; cross-protection; HA2 glycopolypeptide; HA2 ectodomain; fusion peptide; mice; vaccine.
目前，开发具有更广泛疗效谱的流感疫苗的新趋势集中在流感病毒的保守抗原上。HA2糖多肽（HA2 gp）是保守抗原之一，有可能作为诱导针对流感的交叉保护的免疫原。我们从H3亚型甲型流感病毒（IAV）中选择了HA2 gp的两个不同结构域来诱导抗病毒免疫反应：包含第23 - 185位氨基酸的胞外结构域（EHA2）和包含N端第1 - 38位氨基酸的融合肽（FP）。分别用三剂EHA2和FP免疫BALB/c小鼠，随后用同源（H3）或异源（H7）HA亚型的2 LD50 IAV进行攻击。两种肽均诱导了显著的抗体反应，并保护小鼠免受致死性感染。EHA2对同源病毒的保护效果最为显著。
甲型流感病毒；交叉保护；HA2糖多肽；HA2胞外结构域；融合肽；小鼠；疫苗。
