Lubec G, Möschl P
Onkologie. 1979 Aug;2(4):174-5. doi: 10.1159/000214588.
By means of biological in vitro assay collagenolytic activity of normal peripheral lymphocytes was compared to that of EBV-infected lymphoblastoid cell lines: the EBV-producing P3HR1 cells and the non-productive Raji cells. While normal lymphocytes and Raji cells revealed only traces of activity, significantly increased (p less than 0.001) collagenolytic activity was detected in samples of P3HR1, the cell-free EBV-suspension alone showed no collagenolysis. The described enzymatic activity was fully inhibited by 0-phenanthroline and EDTA indicating collagenase action. As main explanations for the phenomenon we suggest the possibility of viral enzyme induction, increased release of protease and thirdly that EBV carries a gene for synthesis of a collagenase.
通过生物体外测定法，将正常外周淋巴细胞的胶原olytic活性与EBV感染的淋巴母细胞系的活性进行了比较：产生EBV的P3HR1细胞和非生产性Raji细胞。虽然正常淋巴细胞和Raji细胞仅显示出微量活性，但在P3HR1样本中检测到胶原olytic活性显著增加（p小于0.001），仅无细胞的EBV悬浮液未显示出胶原分解。所述酶活性被邻菲罗啉和EDTA完全抑制，表明有胶原酶作用。作为该现象的主要解释，我们提出病毒酶诱导的可能性、蛋白酶释放增加，以及第三，EBV携带一种胶原酶合成基因。
