Symbio Alliance, P.O. Box 4312, Eight Mile Plains, Queensland, Australia 4113.
J Food Prot. 2012 Oct;75(10):1862-6. doi: 10.4315/0362-028X.JFP-12-135.
The fourth national baseline microbiological survey of Australian beef was conducted in 2011, including frozen boneless beef and, for the first time, samples from selected beef primal cuts. Cartons of frozen boneless beef (n = 1,165) sampled at 29 boning (fabrication) plants were found to have a mean total viable count of 2.2 log CFU/g, and the mean count for the 2.1% of samples with detectable Escherichia coli was 1.3 log CFU/g. The mean total viable counts for striploins (longissimus dorsi, n = 572) and outsides (biceps femoris, n = 572) were 1.3 and 1.5 log CFU/cm(2) respectively. E. coli isolates were obtained from 10.7 and 25.2% of striploins and outsides, respectively, with mean counts of -0.5 and -0.3 log CFU/cm(2) on positive samples. E. coli O157:H7, Salmonella, and Campylobacter were not isolated from any primal cut samples, and Salmonella was not isolated from any of the boneless product (E. coli O157:H7 and Campylobacter were not tested for). Listeria spp. were not detected in any of the boneless product, and one Listeria isolate was obtained on 1 (0.2%) of 572 striploin samples. Coagulase-positive staphylococci were isolated from 3.4% of boneless beef samples, 7.7% of beef striploins, and 8.4% of beef outsides, with positive samples having mean log counts of 1.9 CFU/g, 0.2 CFU/cm(2), and 0.2 CFU/cm(2), respectively.
2011 年进行了第四次澳大利亚牛肉国家基线微生物调查,其中包括冷冻无骨牛肉,以及首次对选定的牛肉分割部位进行抽样调查。在 29 个剔骨(加工)工厂抽取的冷冻无骨牛肉(n = 1165)样本的总活菌计数平均值为 2.2 log CFU/g,可检测到大肠杆菌的 2.1%样本的平均计数为 1.3 log CFU/g。牛里脊(最长肌,n = 572)和牛臀肉(臀大肌,n = 572)的平均总活菌数分别为 1.3 和 1.5 log CFU/cm(2)。牛里脊和牛臀肉的大肠杆菌分离株分别占 10.7%和 25.2%,阳性样本的平均计数分别为 -0.5 和 -0.3 log CFU/cm(2)。任何分割部位样本均未分离出大肠杆菌 O157:H7、沙门氏菌和弯曲杆菌,也未从任何无骨产品中分离出沙门氏菌(未检测大肠杆菌 O157:H7 和弯曲杆菌)。无骨产品中未检出李斯特菌属,在 572 份牛里脊样本中有 1 份(0.2%)检出李斯特菌。在 3.4%的无骨牛肉样本、7.7%的牛肉里脊和 8.4%的牛肉臀肉中分离到凝固酶阳性葡萄球菌,阳性样本的平均 log 计数分别为 1.9 CFU/g、0.2 CFU/cm(2)和 0.2 CFU/cm(2)。