水麻根汤对慢性应激和高脂血症性脂肪肝大鼠脂代谢相关基因的影响。

Effect of an Ilex asprella root decoction on the related genes of lipid metabolism from chronic stress and hyperlipidemic fatty liver in rats.

机构信息

Department of Traditional Chinese Medicine, Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, China.

出版信息

Chin Med J (Engl). 2012 Oct;125(19):3539-42.

PMID:23044320

Abstract

BACKGROUND

The gradually increasing changes in a human hyperlipidemic diet along with chronic stress might play an important role in the increased numbers of fatty liver. This study investigated the effects of Ilex asprella root decoction on related genes of lipid metabolism in chronic stress in hyperlipidemic fatty liver in rats.

METHODS

Forty-eight male Wistar rats were randomly divided into four groups: normal control group, model control group, simvastatin group, and Ilex asprella root group. To establish chronic stress and hyperlipidemic fatty liver models in rats, the levels of serum lipids, glucose, liver index, insulin (INS), insulin resistant (IR) index, adiponectin, superoxide dismutase (SOD), glutathione peroxidase (GSH-pX), glutathione (GSH), liver X receptor (LXR), and sterol responsive element binding protein (SREBP)-1c in rats were measured.

RESULTS

When compared to the normal control group, the levels of serum lipids, glucose, liver index, INS, IR index, and GSH in the model control group significantly increased (P < 0.01). The protein levels of LXRα and SREBP-1c increased (P < 0.05), and the serum adiponectin and the SOD and GSH-pX decreased significantly (P < 0.01). When compared to the model control group, the levels of serum lipids, glucose, liver index, INS, IR index, SOD, and GSH-pX in the simvastatin group and Ilex asprella root group increased in varying degrees (P < 0.01 or 0.05); the serum adiponectin and GSH decreased (P < 0.05), while the protein levels of LXRα and SREBP-1c decreased in varying degrees (P < 0.01 or 0.05). When compared to the simvastatin group, the IR index and protein levels of LXRα in the Ilex asprella root group decreased (P < 0.05), and the serum adiponectin and SOD increased (P < 0.05).

CONCLUSION

The Ilex asprella root decoction has some protective effects on regulating the related genes of lipid metabolism caused by chronic stress and hyperlipidemic fatty liver in rats.

摘要

背景

人类高血脂饮食的逐渐变化以及慢性应激可能在脂肪肝数量的增加中起着重要作用。本研究探讨了冬青科苦丁茶根煎剂对慢性应激高脂性脂肪肝大鼠脂质代谢相关基因的影响。

方法

48 只雄性 Wistar 大鼠随机分为 4 组：正常对照组、模型对照组、辛伐他汀组、冬青科苦丁茶根组。建立大鼠慢性应激高脂性脂肪肝模型，测定血清脂质、血糖、肝指数、胰岛素（INS）、胰岛素抵抗（IR）指数、脂联素、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-pX）、谷胱甘肽（GSH）、肝 X 受体（LXR）、固醇调节元件结合蛋白-1c（SREBP-1c）在大鼠中的水平。

结果

与正常对照组相比，模型对照组血清脂质、血糖、肝指数、INS、IR 指数和 GSH 水平明显升高（P<0.01）。LXRα 和 SREBP-1c 蛋白水平升高（P<0.05），血清脂联素和 SOD、GSH-pX 水平显著降低（P<0.01）。与模型对照组相比，辛伐他汀组和冬青科苦丁茶根组血清脂质、血糖、肝指数、INS、IR 指数、SOD、GSH-pX 水平均有不同程度的升高（P<0.01 或 0.05）；血清脂联素和 GSH 水平降低（P<0.05），LXRα 和 SREBP-1c 蛋白水平也有不同程度的降低（P<0.01 或 0.05）。与辛伐他汀组相比，冬青科苦丁茶根组 IR 指数和 LXRα 蛋白水平降低（P<0.05），血清脂联素和 SOD 水平升高（P<0.05）。