Pant Kamala, Aardema Marilyn J
BioReliance, Rockville, Maryland, USA.
Curr Protoc Toxicol. 2008 Feb;Chapter 20:Unit20.3. doi: 10.1002/0471140856.tx2003s35.
The Syrian hamster embryonic (SHE) cell transformation assay (CTA) at pH 6.7 can determine the ability of a test article to induce morphological transformation (MT) in cultured SHE cells. The assay uses SHE cells prepared at gestation day ∼13 and frozen. Target cells are seeded onto a layer of feeder cells (X-ray irradiated SHE cells) and treated with test article for 24 hr or 7 days. After a growth period of 7 days, the cells are fixed, stained, and evaluated for MT. Normal colonies typically contain a monolayer of cells with an organized, often flowing, pattern of growth and minimal cell criss-crossing at a confluent density. Transformed colonies contain randomly oriented, stacked cells, with cell criss-crossing throughout the colony. The transformed cells are frequently more basophilic than their normal counterparts, with increased nuclear/cytoplasmic ratios. This assay provides a valuable tool for evaluating the carcinogenic potential of a test article.
叙利亚仓鼠胚胎（SHE）细胞转化试验（CTA）在pH 6.7条件下，可测定受试物诱导培养的SHE细胞发生形态转化（MT）的能力。该试验使用在妊娠约13天时制备并冻存的SHE细胞。将靶细胞接种到一层饲养细胞（经X射线照射的SHE细胞）上，并用受试物处理24小时或7天。经过7天的生长周期后，将细胞固定、染色，并评估其形态转化情况。正常集落通常包含单层细胞，具有有组织的、常呈流动状的生长模式，在汇合密度时细胞交叉极少。转化集落包含随机取向、堆叠的细胞，整个集落中都有细胞交叉。转化细胞通常比正常细胞更嗜碱性，核/质比增加。该试验为评估受试物的致癌潜力提供了一个有价值的工具。
