Morisseau Christophe, Hammock Bruce D
University of California at Davis, Davis, California, USA.
Curr Protoc Toxicol. 2007;Chapter 4:Unit 4.23. doi: 10.1002/0471140856.tx0423s33.
The human soluble epoxide hydrolase (sEH; EC 3.3.3.2) is the product of the EXPH2 gene. The sEH catalyzes the addition of a water molecule to an epoxide, resulting in the corresponding diol. Early work suggested a role of sEH in detoxifying a wide array of xenobiotic epoxides; however, recent findings clearly implicate the sEH in the regulation of blood pressure, pain, and inflammation through the hydrolysis of endogenous epoxy fatty acids such as epoxyeicosatrienoic acids (EETs). Both expression and activity of sEH are influenced by a wide array of xenobiotics, underlying how environmental contaminants could influence human health through sEH. This unit describes radiometric, fluorimetric, and mass spectrometric assays for measuring the activity of sEH and its inhibition.
人可溶性环氧化物水解酶（sEH；EC 3.3.3.2）是EXPH2基因的产物。sEH催化水分子加成到环氧化物上，生成相应的二醇。早期研究表明sEH在多种外源性环氧化物的解毒过程中发挥作用；然而，最近的研究结果明确表明，sEH通过水解内源性环氧脂肪酸，如环氧二十碳三烯酸（EETs），参与血压、疼痛和炎症的调节。sEH的表达和活性受到多种外源性物质的影响，这揭示了环境污染物如何通过sEH影响人类健康。本单元介绍了用于测量sEH活性及其抑制作用的放射性、荧光和质谱分析方法。
