Mulderry P K, Lindsay R M
Cell Biology Laboratory, Sandoz Institute for Medical Research, London, U.K.
Neurosci Lett. 1990 Jan 22;108(3):314-20. doi: 10.1016/0304-3940(90)90660-2.
Expression of vasoactive intestinal polypeptide (VIP) was studied in regenerating adult rat dorsal root ganglion (DRG) neurons in culture. VIP was not detected by radioimmunoassay in newly established cultures but increasing concentrations were observed between 2 and 4 days and VIP remained detectable for more than 2 weeks. Immunocytochemical staining revealed VIP-like immunoreactivity in virtually all neurons. Thus, VIP is expressed in regenerating DRG neurons in culture just as it is in vivo following axotomy. However, VIP concentrations in culture were the same whether nerve growth factor was present or not. Therefore, deprivation of retrogradely transported nerve growth factor is unlikely to explain VIP induction by axotomy in vivo.
对培养的成年大鼠背根神经节（DRG）神经元再生过程中血管活性肠肽（VIP）的表达进行了研究。在新建立的培养物中，放射免疫分析未检测到VIP，但在2至4天之间观察到其浓度不断增加，且VIP在2周多的时间内均可检测到。免疫细胞化学染色显示几乎所有神经元中都有VIP样免疫反应性。因此，VIP在培养的再生DRG神经元中表达，就如同在体内轴突切断后一样。然而，无论是否存在神经生长因子，培养物中的VIP浓度都是相同的。因此，逆行运输的神经生长因子的缺失不太可能解释体内轴突切断诱导VIP产生的原因。
