Department of Biology-Genetics, Philipps-Universität Marburg, Karl-von-Frisch-Str. 8, 35043 Marburg, Germany.
Molecules. 2012 Oct 9;17(10):11920-30. doi: 10.3390/molecules171011920.
Phosphorylation is a key event in many cellular processes like cell cycle, transformation of environmental signals to transcriptional activation or polar growth. The chemical genetics approach can be used to analyse the effect of highly specific inhibition in vivo and is a promising method to screen for kinase targets. We have used this approach to study the role of the germinal centre kinase Don3 during the cell division in the phytopathogenic fungus Ustilago maydis. Due to the easy determination of the don3 phenotype we have chosen this approach for a genetic course for M.Sc. students and for IMPRS (International Max-Planck research school) students. According to the principle of "problem-based learning" the aim of this two-week course is to transfer knowledge about the broad spectrum of kinases to the students and that the students acquire the ability to design their own analog-sensitive kinase of interest. In addition to these training goals, we benefit from these annual courses the synthesis of basic constructs for genetic modification of several kinases in our model system U. maydis.
磷酸化是许多细胞过程中的关键事件，如细胞周期、环境信号向转录激活或极性生长的转化。化学遗传学方法可用于分析体内高度特异性抑制的效果，是筛选激酶靶标的有前途的方法。我们使用这种方法研究了生殖中心激酶 Don3 在植物病原真菌 Ustilago maydis 细胞分裂过程中的作用。由于 don3 表型易于确定，我们选择了这种方法为 M.Sc. 学生和 IMPRS（国际马克斯普朗克研究学院）学生开设了一门遗传学课程。根据“基于问题的学习”原则，这门为期两周的课程的目的是将广泛的激酶知识传授给学生，并使学生获得设计自己感兴趣的模拟敏感激酶的能力。除了这些培训目标外，我们还从这些年度课程中受益于我们的模型系统 U. maydis 中几种激酶的遗传修饰的基本构建体的合成。
