卵泡液中的肉碱含量以及卵母细胞和卵丘细胞中参与β氧化的酶的表达。
Carnitine content in the follicular fluid and expression of the enzymes involved in beta oxidation in oocytes and cumulus cells.
机构信息
UNILABS, Laboratire d'Eylau, 55 Rue St Didier, 75116, Paris, France.
出版信息
J Assist Reprod Genet. 2012 Nov;29(11):1221-5. doi: 10.1007/s10815-012-9855-2. Epub 2012 Oct 3.
Abstract
PURPOSE
The purpose of this study is to study lipid metabolism in oocytes and embryos that is a neglected parameter in human IVF.
METHODS
We have tested the total carnitine content (TC) in the follicular fluid of 278 patients (217 non pregnant, 61 pregnant) undergoing IVF.
RESULTS
The follicular fluid TC is neither correlated with the circulating estradiol content in serum nor with the outcome the IVF attempt. Carnitine, through the carnitine shuttle, is a major partner in lipid beta oxidation, metabolic pathway involved in the acquisition of oocyte competence. The expression of carnitine synthesis enzymes and lipid beta oxidation was studied in cumulus cells collected at the time of ovum retrieval and in oocyte. Surprisingly the expression for carnitine synthesis is not detectable in oocytes whereas the enzymes involved in lipid beta oxidation are rather strongly expressed.
CONCLUSIONS
The addition of carnitine in oocyte maturation and embryo culture media should not be overlooked.
摘要
目的
本研究旨在研究卵母细胞和胚胎中的脂质代谢，这是人类体外受精中被忽视的一个参数。
方法
我们检测了 278 名接受体外受精的患者（217 名未怀孕，61 名怀孕）的卵泡液中的总肉碱含量（TC）。
结果
卵泡液 TC 既与血清中环化雌二醇含量无关，也与体外受精尝试的结果无关。肉碱通过肉碱穿梭，是脂类β氧化的主要伴侣，脂类β氧化代谢途径参与卵母细胞获得能力。在取卵时收集的卵丘细胞和卵母细胞中研究了肉碱合成酶和脂类β氧化的表达。令人惊讶的是，在卵母细胞中检测不到肉碱合成的表达，而参与脂类β氧化的酶则强烈表达。
结论
在卵母细胞成熟和胚胎培养介质中添加肉碱不应被忽视。
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