UPASI-Tea Research Foundation, Nirar Dam B.P.O, Valparai, 642127 Tamil Nadu, India.
Appl Biochem Biotechnol. 2012 Dec;168(7):1907-16. doi: 10.1007/s12010-012-9906-2. Epub 2012 Oct 11.
Nucleoside diphosphate kinase (NDPK, EC 2.7.4.6) is a housekeeping gene, which functions in the general homeostasis of cellular nucleoside triphosphate (NTP) pools. Among the various NDPK isoforms, cytosolic NDPK1 has been shown to be the main NDPK isoform in plants, accounting for more than 70 % of total NDPK activity in plants. For the first time, a full-length cDNA (697 bp), designated as CsNDPK1 was cloned from tea leaves and consisted of a 448-bp open reading frame (ORF) encoding a 147-amino-acid polypeptide with calculated molecular mass of 16.1 kDa and a pI of 6.3. Homology modeling of CsNDPK1 shows that the presented tea NDPK1 also contains several motifs, binding and catalytic sites which are highly conserved among other NDPKs. Docking studies of CsNDPK1 with its substrates (NTPs) are discussed in detail. In summary, we describe a reliable model of CsNDPK1 that can be used in structure-based protein-protein interaction studies for identifying its potential role in intracellular communication and its physiological significance in tea.
核苷二磷酸激酶（NDPK，EC 2.7.4.6）是一种管家基因，其功能是维持细胞核苷三磷酸（NTP）池的一般动态平衡。在各种 NDPK 同工酶中，细胞质 NDPK1 已被证明是植物中的主要 NDPK 同工酶，占植物中总 NDPK 活性的 70%以上。首次从茶叶中克隆出全长 cDNA（697bp），命名为 CsNDPK1，由 448bp 的开放阅读框（ORF）编码，编码一个 147 个氨基酸的多肽，计算分子量为 16.1kDa，等电点为 6.3。CsNDPK1 的同源建模表明，该茶 NDPK1 还包含几个基序、结合和催化位点，这些基序在其他 NDPKs 中高度保守。详细讨论了 CsNDPK1 与底物（NTP）的对接研究。总之，我们描述了一个可靠的 CsNDPK1 模型，可用于基于结构的蛋白质-蛋白质相互作用研究，以确定其在细胞内通讯中的潜在作用及其在茶中的生理意义。
