Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Appl Biochem Biotechnol. 2012 Dec;168(8):2184-90. doi: 10.1007/s12010-012-9928-9. Epub 2012 Oct 11.
Stable carriers are required for gene delivery. The use of polyethyleneimine (PEI) has been researched extensively; however, it is not suitable for targeted gene delivery to specific cells. To provide a targeting ability of the specific antibody to PEI, two repeats of Fc-binding domain of protein G (C2) were utilized. The constructed protein containing C2 could bind to a specific antibody and form a larger complex with plasmid DNA/PEI complex. The specific antibody to cell surface protein was bound to the complex through the use of C2 domain, and it was added to cells. As the result, the efficient expression of delivered reporter gene could be realized.
稳定载体是基因递送所必需的。聚亚乙基亚胺（PEI）的应用已得到广泛研究；然而，它并不适合针对特定细胞的靶向基因递送。为了提供针对特定抗体的靶向能力，利用了蛋白 G（C2）的两个 Fc 结合结构域重复。构建的包含 C2 的蛋白可以与特定抗体结合，并与质粒 DNA/PEI 复合物形成更大的复合物。通过使用 C2 结构域，将针对细胞表面蛋白的特异性抗体结合到复合物上，并将其添加到细胞中。结果，可以实现递送到的报告基因的高效表达。
