Université Bordeaux Segalen, Interdisciplinary Institute for Neuroscience, UMR 5297, 146 rue Léo Saignat, 33077 Bordeaux, France.
Exp Neurol. 2013 Apr;242:33-40. doi: 10.1016/j.expneurol.2012.10.004. Epub 2012 Oct 11.
The advent of superresolution fluorescence microscopy beyond the classic diffraction barrier of optical microscopy is poised to transform cell-biological research. A series of proof-of-principle studies have demonstrated its vast potential for a wide range of applications in neuroscience, including nanoscale imaging of neuronal morphology, cellular organelles, protein distributions and protein trafficking. This review introduces the main incarnations of these new methodologies, including STED, PALM/STORM and SIM, covering basic theoretical and practical aspects concerning their optical principles, technical implementation, scope and limitations. In addition, it highlights several discoveries relating to synapse biology that have been made using these novel approaches to illustrate their appeal for neuroscience research.
超分辨率荧光显微镜的出现突破了传统光学显微镜的衍射极限，有望彻底改变细胞生物学研究。一系列原理验证研究已经证明，它在神经科学的多个领域都具有广泛的应用潜力，包括对神经元形态、细胞细胞器、蛋白质分布和蛋白质运输的纳米级成像。本文介绍了这些新技术方法的主要形式，包括 STED、PALM/STORM 和 SIM，涵盖了它们的光学原理、技术实现、应用范围和局限性等基本理论和实际方面。此外，本文还强调了利用这些新方法在突触生物学方面的一些发现，以说明它们对神经科学研究的吸引力。
