Department of Physiology, Nanchang, Jiangxi 330006, PR China.
Brain Res Bull. 2013 Jan;90:79-87. doi: 10.1016/j.brainresbull.2012.10.002. Epub 2012 Oct 9.
Chemokines and their receptors have the key role in inflammatory responses. The phenomenon of low grade inflammation is associated with the development of type 2 diabetes. Postprandial hyperglycemia increases the systemic inflammatory responses, which promotes the development of type 2 diabetic associating autonomic nervous injuries or cardiovascular disease. Neferine is a bisbenzylisoquinline alkaloid isolated from a Chinese medicinal herb. The objectives of this study will examine the CCL5 and CCR5 expression in the superior cervical ganglion (SCG) of type 2 diabetic rats. The effects of neferine on the expression of CCL5 and CCR5 mRNA and protein in the superior cervical ganglion (SCG) of type 2 diabetic rats will also be observed. The studies showed that in type 2 diabetic rats, body weight, blood pressure, heart rates, fasting blood glucose, insulin, total cholesterol and triglyceride were enhanced and high density lipoprotein was decreased, and CCL5 and CCR5 expression levels in the SCG of type 2 diabetic rats were up-regulated. In type 2 diabetic rats treated with neferine, body weight, blood pressure, fasting blood glucose, insulin, total cholesterol and triglyceride were decreased and high density lipoprotein was increased. The elevated expressions of CCL5 and CCR5 in SCG were decreased after type 2 diabetic rats treated with neferine. The motor nerve conduction velocity (MNCV) in diabetic rats treated with neferine group showed a significantly increment in comparison with that in type 2 diabetic group. Neferine can decrease the expression of CCL5 and CCR5 in the SCG and reduce the SCG neuronal signaling mediated by CCL5 and CCR5 in regulating diabetic cardiovascular autonomic complications.
趋化因子及其受体在炎症反应中起着关键作用。低度炎症现象与 2 型糖尿病的发展有关。餐后高血糖会增加全身炎症反应，从而促进 2 型糖尿病相关自主神经损伤或心血管疾病的发展。甲基莲心碱是从一种中药中分离得到的双苄基异喹啉生物碱。本研究的目的是观察 2 型糖尿病大鼠颈上神经节（SCG）中 CCL5 和 CCR5 的表达。观察甲基莲心碱对 2 型糖尿病大鼠颈上神经节（SCG）中 CCL5 和 CCR5 mRNA 和蛋白表达的影响。研究表明，在 2 型糖尿病大鼠中，体重、血压、心率、空腹血糖、胰岛素、总胆固醇和甘油三酯升高，高密度脂蛋白降低，2 型糖尿病大鼠 SCG 中 CCL5 和 CCR5 的表达水平上调。在接受甲基莲心碱治疗的 2 型糖尿病大鼠中，体重、血压、空腹血糖、胰岛素、总胆固醇和甘油三酯降低，高密度脂蛋白升高。与 2 型糖尿病组相比，接受甲基莲心碱治疗的糖尿病大鼠 SCG 中 CCL5 和 CCR5 的表达升高。与 2 型糖尿病组相比，接受甲基莲心碱治疗的糖尿病大鼠的运动神经传导速度（MNCV）明显增加。甲基莲心碱可降低 SCG 中 CCL5 和 CCR5 的表达，降低 CCL5 和 CCR5 介导的 SCG 神经元信号在调节糖尿病心血管自主神经并发症中的作用。
