Rao Shenqiang, Liu Shuangmei, Zou Lifang, Jia Tianyu, Zhao Shanhong, Wu Bing, Yi Zhihua, Wang Shouyu, Xue Yun, Gao Yun, Xu Changshui, Li Guilin, Xu Hong, Zhang Chunping, Liang Shangdong
Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
Purinergic Signal. 2017 Jun;13(2):227-235. doi: 10.1007/s11302-016-9554-z. Epub 2017 Jan 4.
Type 2 diabetes mellitus (T2DM) accounts for more than 90% of all cases of diabetes mellitus (DM). Diabetic neuropathic pain (DNP) is a common complication of T2DM. Sinomenine is a natural bioactive component extracted from the Sinomenium acutum and has anti-inflammatory effects. The aim of our study was to investigate the effects of sinomenine on DNP mediated by the P2X receptor in dorsal root ganglia (DRG). The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) in T2DM rats were lower than those of control rats. MWT and TWL in T2DM rats treated with sinomenine were higher compared with those in T2DM rats. The expression levels of the P2X protein and mRNA in T2DM rat DRG were higher compared with those of the control, while those in T2DM rats treated with sinomenine were significantly lower compared with those of the T2DM rats. Sinomenine significantly inhibited P2X agonist ATP-activated currents in HEK293 cells transfected with the P2X receptor. Sinomenine decreased the phosphorylation and activation of P38MAPK in T2DM DRG. Therefore, sinomenine treatment may suppress the up-regulated expression and activation of the P2X receptor and relieve the hyperalgesia potentiated by the activation of P38MAPK in T2DM rats.
2型糖尿病（T2DM）占所有糖尿病（DM）病例的90%以上。糖尿病性神经病理性疼痛（DNP）是T2DM的常见并发症。青藤碱是从青风藤中提取的一种天然生物活性成分，具有抗炎作用。我们研究的目的是探讨青藤碱对背根神经节（DRG）中P2X受体介导的DNP的影响。T2DM大鼠的机械缩足阈值（MWT）和热缩足潜伏期（TWL）低于对照大鼠。与T2DM大鼠相比，用青藤碱治疗的T2DM大鼠的MWT和TWL更高。与对照组相比，T2DM大鼠DRG中P2X蛋白和mRNA的表达水平更高，而与T2DM大鼠相比，用青藤碱治疗的T2DM大鼠的表达水平显著更低。青藤碱显著抑制转染了P2X受体的HEK293细胞中P2X激动剂ATP激活的电流。青藤碱降低了T2DM DRG中P38MAPK的磷酸化和激活。因此，青藤碱治疗可能会抑制T2DM大鼠中P2X受体的上调表达和激活，并减轻由P38MAPK激活所增强的痛觉过敏。
