College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Mol Biol Rep. 2013 Feb;40(2):1087-95. doi: 10.1007/s11033-012-2151-5. Epub 2012 Oct 14.
The peritrophic membrane lines the gut of most insects at one or more stages of their life cycles. It facilitates the digestive processes in the guts and protects from invasion by pathogens or food particles. In the current study, a novel PM protein, designated as BmMtch, was identified from the silkworm, Bombyx mori. The open reading frame of BmMtch is 888 bp in length, encoding 295 amino acid residues consisting of two domains (Mito_carr domains) and three transmembrane regions. They are localized on the 11th chromosome as single copy with one exon only. Quantitative real time PCR analysis (qRT-PCR) revealed that BmMtch was mainly expressed in larval fat bodies, Malpighian tubules, testis and ovaries, and could be detected through all stages of the life cycle of silkworm. Immuno-fluorescence analysis indicated that BmMtch was localized within the goblet cell of larval midgut. Western blotting analysis showed that BmMtch were detected in total proteins of PM and larval midgut. The characteristics of BmMtch indicated that BmMtch represents a novel member of insect PM proteins, without chitin-binding domains.
围食膜存在于大多数昆虫生命周期的一个或多个阶段的肠道中。它有助于肠道中的消化过程，并防止病原体或食物颗粒的入侵。在本研究中，从家蚕（Bombyx mori）中鉴定出一种新型 PM 蛋白，命名为 BmMtch。BmMtch 的开放阅读框长 888bp，编码由两个结构域（Mito_carr 结构域）和三个跨膜区组成的 295 个氨基酸残基。它们作为单拷贝存在于第 11 号染色体上，只有一个外显子。定量实时 PCR 分析（qRT-PCR）显示，BmMtch 主要在幼虫脂肪体、马氏管、睾丸和卵巢中表达，并可在蚕的整个生命周期中检测到。免疫荧光分析表明，BmMtch 定位于幼虫中肠的杯状细胞内。Western blotting 分析显示，BmMtch 存在于 PM 和幼虫中肠的总蛋白中。BmMtch 的特征表明，BmMtch 代表昆虫 PM 蛋白的一个新成员，没有几丁质结合结构域。
