Department of Chemistry, Indian Institute of Technology, Patna 800013, Bihar, India.
Photochem Photobiol Sci. 2013 Feb;12(2):369-83. doi: 10.1039/c2pp25297j. Epub 2012 Oct 15.
The photophysics of thioflavin T (ThT) were observed in different non-aqueous reverse micelles by using steady state absorption and emission spectroscopy and time resolved fluorescence spectroscopy. We have used glycerol, ethylene glycol, dimethyl formamide, methanol and acetonitrile as polar solvents to form reverse micelles. In all reverse micelles with increase in w (w = [polar solvent]/[AOT]) the fluorescence quantum yield decreased in a regular way. The time resolved fluorescence study of ThT in different reverse micelles shows the similar trend as like above. We had plotted a calibration curve using the fluorescence quantum yield of ThT in glycerol-methanol mixtures vs. viscosity and from that curve we found that ThT faced a greater microviscosity in glycerol pool of AOT/isooctane/glycerol reverse micelles than in the pure glycerol, even at the highest w value. In all reverse micelles the emission quantum yield of ThT was retarded several times compared to neat solvents. We have found that the fluorescence quantum yield of ThT decreased with gradual increase in polarity by studying the emission properties of ThT in dioxane-water mixtures of different polarity.
通过稳态吸收和发射光谱以及时间分辨荧光光谱，在不同的非水反胶束中观察了噻吨酮（ThT）的光物理性质。我们使用甘油、乙二醇、二甲基甲酰胺、甲醇和乙腈作为极性溶剂来形成反胶束。在所有反胶束中，随着 w 值（w = [极性溶剂]/[AOT]）的增加，荧光量子产率呈规则下降。ThT 在不同反胶束中的时间分辨荧光研究显示出与上述相似的趋势。我们使用 ThT 在甘油-甲醇混合物中的荧光量子产率与粘度的关系绘制了校准曲线，从该曲线我们发现，即使在最高 w 值下，ThT 在 AOT/异辛烷/甘油反胶束中的甘油池中的微观粘度也大于纯甘油。在所有反胶束中，与纯溶剂相比，ThT 的发射量子产率延迟了几倍。通过研究 ThT 在不同极性的二氧六环-水混合物中的发射性质，我们发现随着极性的逐渐增加，ThT 的荧光量子产率降低。
