Departamento de Fisiología, Facultad de Medicina, Granada, Spain.
Hypertension. 2012 Dec;60(6):1458-63. doi: 10.1161/HYPERTENSIONAHA.112.198499. Epub 2012 Oct 15.
We investigated the effects of uninephrectomy (UNX) in 6-week-old male and female rats on blood pressure (BP), renal sodium handling, salt sensitivity, oxidative stress, and renal injury over 18 months postsurgery, studying control sham-operated and UNX-operated rats at 6, 12, and 18 months postsurgery, evaluating their renal sodium handling, BP, urinary isoprostanes, N-acetyl-β-D-glucosaminidase, and proteinuria before and after a 2-week high-salt intake period. At 18 months, plasma variables were measured and kidney samples were taken for the analysis of renal morphology and tissue variables. BP was increased at 6 months in male UNX rats versus controls and at 12 and 18 months in both male and female UNX rats and was increased in male versus female UNX groups at 18 months. UNX did not affect water and sodium excretion under basal conditions and after the different test in male and female rats at different ages. However, the renal function curve was shifted to the right in both male and female UNX rats. High-salt intake increased BP in both UNX groups at 6, 12, and 18 months and in the female control group at 18 months, and it increased proteinuria, N-acetyl-β-D-glucosaminidase, and isoprostanes in both UNX groups throughout the study. Renal lesions at 18 months were more severe in male versus female UNX rats. In summary, long-term UNX increased the BP, creatinine, proteinuria, pathological signs of renal injury, and salt sensitivity. Earlier BP elevation was observed and morphological lesions were more severe in male than in female UNX rats.
我们研究了单侧肾切除术（UNX）对 6 周龄雄性和雌性大鼠血压（BP）、肾脏钠处理、盐敏感性、氧化应激和肾损伤的影响，在手术后 18 个月内研究了对照假手术和 UNX 手术的大鼠，并在手术后 6、12 和 18 个月评估了它们的肾脏钠处理、BP、尿异前列烷、N-乙酰-β-D-氨基葡萄糖苷酶和蛋白尿，在 2 周高盐摄入期前后。在 18 个月时，测量了血浆变量，并采集了肾脏样本，用于分析肾脏形态和组织变量。与对照组相比，雄性 UNX 大鼠的 BP 在 6 个月时升高，在 12 个月和 18 个月时雄性和雌性 UNX 大鼠的 BP 均升高，且雄性 UNX 大鼠的 BP 高于雌性 UNX 大鼠。UNX 不影响雄性和雌性大鼠在不同年龄时的基础条件和不同测试下的水和钠排泄。然而，肾脏功能曲线在雄性和雌性 UNX 大鼠中均向右侧移位。高盐摄入增加了 6、12 和 18 个月时 UNX 组的 BP，以及 18 个月时雌性对照组的 BP，并增加了 UNX 组在整个研究中的蛋白尿、N-乙酰-β-D-氨基葡萄糖苷酶和异前列烷。与雌性 UNX 大鼠相比，18 个月时雄性 UNX 大鼠的肾脏病变更严重。总之，长期 UNX 增加了 BP、肌酐、蛋白尿、肾脏损伤的病理迹象和盐敏感性。在雄性 UNX 大鼠中观察到更早的 BP 升高，且形态学病变比雌性 UNX 大鼠更严重。
