Wu Zhao-Li, Li Chun-Ri, Liu Zi-Li, Zhang Qing-Rong
College of Acupuncture-Moxibustion and Massage, Liaoning University of TCM, Shenyang 110847, China.
Zhongguo Zhen Jiu. 2012 Aug;32(8):721-5.
To observed efficacy differences of acupuncture at "Zusanli" (ST 36) in rats with asthma and asthma with spleen-deficency, so as to investigate the therapeutic mechanism.
Sixty SD rats were randomly divided into 5 groups according to their weight, named as an asthma with spleen-deficency group (group A), an acupuncture on asthma with spleen-deficency group (group B), an asthma group (group C), an acupuncture on asthma group (group D) and a control group. The rat models with spleen-deficiency in the first two groups were set up by TCM, then the rats of asthma model in the first four groups were induced by egg albumin, but the control group was treated by the same dose of saline. The group B and the group D were both treated with acupuncture at "Zusanli" (ST 36), once each day for 8 days, and the other groups remained unhandled. The mRNA expressions of Fas and Bcl-2 in the lung tissues were detected by hybridization in situ and apoptosis was detected by TUNEL (terminal dexynucleotidyl transferase-mediated dutp nick end labeling).
Compared with the control group, in both the group A and the group C, the expression of Fas mRNA significantly decreased, but the expression of Bcl-2 mRNA significantly increased (all P < 0.01), and eosinophils (EOS) counts significantly increased, but EOS apoptosis rate significantly decreased (all P < 0.01). Compared with the group C, in the group A, the expressions of Fas mRNA significantly decreased, but the expressions of Bcl-2 mRNA and EOS counts significantly increased (all P < 0.01). At the same time, compared with the corresponding asthma groups, in both acupuncture groups, Fas mRNA expression obviously increased, Bcl-2 mRNA expression was significantly reduced (all P < 0.01), EOS counts remarkably decreased and EOS apoptosis rate significantly increased (all P < 0.01). There were no significant differences in the expressions of Fas mRNA and Bcl-2 mRNA between the two acupuncture groups (both P > 0.05), but compared with group B,in the group D, EOS counts significantly decreased and EOS apoptosis rate significantly increased (both P < 0.01).
Acupuncture at "Zusanli" (ST 36) can regulate the disorders of Fas mRNA and Bcl-2 mRNA expression in the state of both asthma and asthma with spleen-deficency, promote EOS apoptosis so as to inhibit the development of inflammatory reaction of asthma, showing that acupuncture at "Zusanli" (ST 36) has certain advantages on regulation of related gene of EOS in asthma with spleen-deficency.
观察针刺足三里穴对哮喘及脾虚哮喘大鼠的疗效差异，探讨其治疗机制。
60只SD大鼠按体重随机分为5组，分别为脾虚哮喘组（A组）、针刺脾虚哮喘组（B组）、哮喘组（C组）、针刺哮喘组（D组）和正常对照组。前两组脾虚大鼠模型采用中医方法建立，后四组哮喘模型大鼠采用卵白蛋白诱导，正常对照组给予等量生理盐水。B组和D组均针刺足三里穴，每日1次，连续8天，其余各组不予处理。采用原位杂交法检测肺组织中Fas和Bcl-2 mRNA表达，TUNEL法检测细胞凋亡。
与正常对照组比较，A组和C组Fas mRNA表达均明显降低，Bcl-2 mRNA表达均明显升高（均P<0.01），嗜酸性粒细胞（EOS）计数均明显增加，EOS凋亡率均明显降低（均P<0.01）。与C组比较，A组Fas mRNA表达明显降低，Bcl-2 mRNA表达及EOS计数明显升高（均P<0.01）。同时，与相应哮喘组比较，针刺两组Fas mRNA表达明显升高，Bcl-2 mRNA表达明显降低（均P<0.01），EOS计数明显减少，EOS凋亡率明显升高（均P<0.01）。针刺两组Fas mRNA和Bcl-2 mRNA表达差异无统计学意义（均P>0.05），但与B组比较，D组EOS计数明显减少，EOS凋亡率明显升高（均P<0.01）。
针刺足三里穴可调节哮喘及脾虚哮喘状态下Fas mRNA和Bcl-2 mRNA表达紊乱，促进EOS凋亡，从而抑制哮喘炎症反应的发展，提示针刺足三里穴对脾虚哮喘EOS相关基因的调控有一定优势。
