Yang Hong-Qiang, Ma Shao-Bo, Bian Zhi-Yuan, Li Jiang, Zou Hong, Zhang Shi-Jie, Peng Xin-Yu, Chen Xiao-Ping
Department of Hepatobiliary Surgery, the First Affiliated Hospital, Medical College of Shihezi University, Shihezi 832002, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2012 Jun 30;30(3):201-5.
To investigate the expression and its significance of tumor necrosis factor-alpha (TNF-alpha) and caspase-3 protein in monocytes adjacent to the invaded Echinococcus multilocularis in liver.
40 female Kunming mice were randomly divided into experimental group (n=20) and sham operation (control) group (n=20). Mice in experimental group were infected with 20% E. multilocularis suspension (0.1 ml per mouse) through abdominal opening injection in liver and the mice in control group were injected with equal physiological saline. The mice were sacrificed at 6 months post-infection for observing the growth and metastasis of E. multilocularis. Pathological changes were observed by HE staining. The expression of TNF-alpha and caspase-3 protein in hydatid cyst and metastasis tissue were detected by immunohistochemistry staining and the apoptosis of the monocytes was measured by TUNEL.
After 6 months post-infection, E. multilocularis were spread over the liver of the mice in experimental group. Metastasis rate of lymph nodes was 45.0% (9/20). Infiltration of monocytes was observed around E. multilocularis in liver and lymph nodes with metastasis by HE staining. Immunohistochemistry showed that the positive expression rate of TNF-alpha and caspase-3 protein in monocytes was 100% and 100%, and 95% and 100% respectively around the cyst in experimental group, while the expression rate was only 5% and 0 respectively in the liver of the control mice (P<0.01). The monocytes showed significant apoptosis by TUNEL in experimental group with a positive expression rate of 100%, with a significant difference between experiment group and the control (P<0.01).
In the process of alveolar Echinococcus infection, the high expression of TNF-alpha protein might be associated with the apoptosis of monocytes, which may inhibit the host immunological function.
探讨肝内多房棘球蚴侵袭部位附近单核细胞中肿瘤坏死因子-α（TNF-α）和半胱天冬酶-3蛋白的表达及其意义。
将40只雌性昆明小鼠随机分为实验组（n=20）和假手术（对照）组（n=20）。实验组小鼠经肝切开注射20%多房棘球蚴悬液（每只小鼠0.1 ml），对照组小鼠注射等量生理盐水。感染后6个月处死小鼠，观察多房棘球蚴的生长和转移情况。采用HE染色观察病理变化。通过免疫组织化学染色检测包虫囊肿及转移组织中TNF-α和半胱天冬酶-3蛋白的表达，采用TUNEL法检测单核细胞的凋亡情况。
感染后6个月，实验组小鼠肝脏内多房棘球蚴广泛播散，淋巴结转移率为45.0%（9/20）。HE染色显示，在肝脏和有转移的淋巴结中，多房棘球蚴周围可见单核细胞浸润。免疫组织化学结果显示，实验组囊肿周围单核细胞中TNF-α和半胱天冬酶-3蛋白的阳性表达率分别为100%和100%，95%和100%，而对照组小鼠肝脏中表达率分别仅为5%和0（P<0.01）。实验组TUNEL法检测显示单核细胞有明显凋亡，阳性表达率为100%，实验组与对照组差异有统计学意义（P<0.01）。
在泡型棘球蚴感染过程中，TNF-α蛋白的高表达可能与单核细胞凋亡有关，进而可能抑制宿主免疫功能。
