Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan.
Plant Signal Behav. 2012 Dec;7(12):1648-52. doi: 10.4161/psb.22294. Epub 2012 Oct 16.
Water homeostasis is crucial to the growth and survival of plants. Plasma membrane intrinsic proteins (PIPs) have been shown to be primary channels mediating water uptake in plant cells. We characterized a novel PIP2 gene, HvPIP2;8 in barley (Hordeum vulgare). HvPIP2;8 shared 72-76% identity with other HvPIP2s and 74% identity with rice OsPIP2;8. The gene was expressed in all organs including the shoots, roots and pistil at a similar level. When HvPIP2;8 was transiently expressed in onion epidermal cells, it was localized to the plasma membrane. HvPIP2;8 showed transport activity for water in Xenopus oocytes, however its interaction with HvPIP1;2 was not observed. These results suggest that HvPIP2;8 plays a role in water homeostasis although further functional analysis is required in future.
水稳态对植物的生长和存活至关重要。质膜内在蛋白（PIPs）已被证明是介导植物细胞水分吸收的主要通道。我们在大麦（Hordeum vulgare）中鉴定了一个新的 PIP2 基因 HvPIP2;8。HvPIP2;8 与其他 HvPIP2 具有 72-76%的同源性，与水稻 OsPIP2;8 具有 74%的同源性。该基因在所有器官中均有表达，包括芽、根和雌蕊，表达水平相似。当 HvPIP2;8 在洋葱表皮细胞中瞬时表达时，它定位于质膜上。HvPIP2;8 在非洲爪蟾卵母细胞中显示出对水的转运活性，但未观察到其与 HvPIP1;2 的相互作用。这些结果表明，尽管需要进一步的功能分析，但 HvPIP2;8 在水稳态中发挥作用。
