MOE Key Laboratory of Aquatic Product Safety/State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China.
Virus Res. 2013 Jan;171(1):8-14. doi: 10.1016/j.virusres.2012.10.002. Epub 2012 Oct 13.
A virus with a particle diameter of approximately 30 nm and no envelope was purified from diseased mud crab, Scylla paramamosain and it was demonstrated to be pathogenic to mud crab. The complete nucleotide sequence analysis indicated that its genome was a single molecule of linear positive-sense ssRNA with a length of 10,415 nucleotides, excluding the 3'poly (A) tail. It consisted of two open reading frames (ORF) separated by an intergenic region (IGR) and flanked by a 5'untranslated region (5'-UTR) and a 3'untranslated region (3'-UTR). The 5'-ORF encode five putative non-structural proteins, including BIR (Baculovirus Inhibitor of Apoptosis Protein Repeat), helicase, VPg (the genome-linked viral protein), 3C-like protease and RdRP (RNA-dependent RNA polymerase), while the 3'-ORF encode the structural protein precursors. This genome organization was consistent with the typical organization of dicistrovirus and the virus was designated as mud crab dicistrovirus-1 (MCDV-1). The results of the phylogenetic analysis of the putative structural protein precursor suggest that MCDV-1 has a closer genetic relationship with Taura syndrome virus (TSV) than do other dicistroviruses and that MCDV-1 is a new member of the family Dicistroviridae and assigned into the genus Aparavirus.
从患病的泥蟹（Scylla paramamosain）中纯化出一种直径约 30nm、无包膜的病毒，证明其对泥蟹具有致病性。完整的核苷酸序列分析表明，其基因组是一条长 10415 个核苷酸的单分子线性正义 ssRNA，不包括 3'poly(A)尾巴。它由两个开放阅读框（ORF）组成，由一个基因间区（IGR）隔开，两端分别为 5'非翻译区（5'-UTR）和 3'非翻译区（3'-UTR）。5'-ORF 编码五个假定的非结构蛋白，包括 BIR（凋亡蛋白重复的杆状病毒抑制剂）、解旋酶、VPg（基因组连接的病毒蛋白）、3C 样蛋白酶和 RdRP（RNA 依赖性 RNA 聚合酶），而 3'-ORF 编码结构蛋白前体。这种基因组组织与双顺反子病毒的典型组织一致，该病毒被命名为泥蟹双顺反子病毒-1（MCDV-1）。假定结构蛋白前体的系统发育分析结果表明，与其他双顺反子病毒相比，MCDV-1 与 Taura 综合征病毒（TSV）具有更密切的遗传关系，并且 MCDV-1 是双顺反子病毒科的一个新成员，并归入 Aparavirus 属。
