Department of Animal Sciences, University of Illinois, Urbana, Illinois 61801, United States.
J Proteome Res. 2012 Dec 7;11(12):5924-33. doi: 10.1021/pr3006809. Epub 2012 Nov 5.
Four healthy adult cats were used in a crossover design to determine phylogeny and metabolic functional capacity of the cat's gastrointestinal microbiota using a metagenomic approach. Healthy adult cats (1.7 years old) were fed diets containing 4% cellulose, fructooligosaccharides (FOS), or pectin for 30 d, at which time fresh fecal samples were collected. Fecal DNA samples from each cat consuming each diet were subjected to 454 pyrosequencing. Dominant phyla determined using two independent databases (MG-RAST and IMG/M) included Firmicutes (mean=36.3 and 49.8%, respectively), Bacteroidetes (mean=36.1 and 24.1%, respectively), and Proteobacteria (mean=12.4 and 11.1%, respectively). Primary functional categories as determined by KEGG were associated with carbohydrates, clustering-based subsystems, protein metabolism, and amino acids and derivatives. Primary functional categories as determined by COG were associated with amino acid metabolism and transport, general function prediction only, and carbohydrate transport and metabolism. Analysis of carbohydrate-active enzymes revealed modifications in several glycoside hydrolases, glycosyl transferases, and carbohydrate-binding molecules with FOS and pectin consumption. While the cat is an obligate carnivore, its gut microbiome is similar regarding microbial phylogeny and gene content to omnivores.
采用宏基因组学方法，利用交叉设计，选择 4 只健康成年猫来确定猫的胃肠道微生物区系的系统发育和代谢功能能力。健康成年猫（1.7 岁）食用含 4%纤维素、果寡糖（FOS）或果胶的饮食 30 天，此时收集新鲜粪便样本。每只猫摄入每种饮食的粪便 DNA 样本均进行 454 焦磷酸测序。使用两个独立的数据库（MG-RAST 和 IMG/M）确定的主要菌群包括厚壁菌门（平均值分别为 36.3%和 49.8%）、拟杆菌门（平均值分别为 36.1%和 24.1%）和变形菌门（平均值分别为 12.4%和 11.1%）。KEGG 确定的主要功能类别与碳水化合物、基于聚类的子系统、蛋白质代谢以及氨基酸和衍生物有关。COG 确定的主要功能类别与氨基酸代谢和转运、一般功能预测和碳水化合物转运和代谢有关。碳水化合物活性酶分析表明，FOS 和果胶的消耗会导致几种糖苷水解酶、糖基转移酶和碳水化合物结合分子发生修饰。虽然猫是一种专性肉食动物，但它的肠道微生物组在微生物系统发育和基因组成方面与杂食动物相似。
