克隆和功能验证编码咪唑甘油磷酸脱水酶的米勒毕赤酵母 HIS3 基因。

Cloning and functional verification of the Candida milleri HIS3 gene encoding imidazoleglycerol phosphate dehydratase.

机构信息

Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.

出版信息

J Microbiol Biotechnol. 2012 Oct;22(10):1441-5. doi: 10.4014/jmb.1207.07064.

DOI:10.4014/jmb.1207.07064

Abstract

The entire nucleotide sequence of the HIS3 gene encoding imidazoleglycerol phosphate dehydratase (IGPD) in yeast Candida milleri CBS 8195 was determined. Sequence analysis revealed an open-reading frame of C. milleri HIS3 that spans 678 bp, encodes 226 amino acids, and shares 80.5% amino acid identity to Torulaspora delbrueckii IGPD, followed by that to Saccharomyces cerevisiae (79.6%). The cloned HIS3 gene complemented a his3 mutation in S. cerevisiae, suggesting that it encodes a functional IGPD in C. milleri CBS 8195. A new auxotrophic marker is now available for acid-tolerant yeast C. milleri.

摘要

测定了产朊假丝酵母 CBS8195 编码组氨酸磷酸甘油脱水酶（IGPD）的 HIS3 基因的全核苷酸序列。序列分析揭示了一个开放阅读框，该阅读框跨越 678bp，编码 226 个氨基酸，与德尔布吕克毕赤酵母的 IGPD 有 80.5%的氨基酸同一性，其次是酿酒酵母（79.6%）。克隆的 HIS3 基因可补充酿酒酵母中的 his3 突变，表明它在 CBS8195 中编码一个功能性的 IGPD。现在，一种新的营养缺陷型标记物可用于耐酸酵母产朊假丝酵母。