Center for Oncology and Cell Biology, The Feinstein Institute for Medical Research at North Shore-LIJ, Manhasset, NY, USA.
Cell Adh Migr. 2012 Nov-Dec;6(6):518-25. doi: 10.4161/cam.22139. Epub 2012 Oct 17.
Synaptojanin 2 (SYNJ2) is a phosphatidylinositol (PI) phosphatase that controls two distinct functions, clathrin-mediated endocytosis and tumor cell invadopodia formation and invasion. Here, we identify a number of novel SYNJ2 binding partners, several of which have previously been shown to be necessary for invadopodia formation or clathrin-mediated endocytosis. We focus on Src family kinases. We found that Src phosphorylates SYNJ2 on Tyr ( 490) , thereby stimulating SYNJ2 5'-phosphatase activity in vitro. We also provide evidence that Src-mediated phosphorylation of SYNJ2 contributes to invadopodia formation.
突触结合蛋白 2（SYNJ2）是一种磷酸肌醇（PI）磷酸酶，它控制着两个不同的功能，网格蛋白介导的内吞作用和肿瘤细胞侵袭伪足的形成和侵袭。在这里，我们鉴定了一些新的 SYNJ2 结合伙伴，其中一些以前被证明是侵袭伪足形成或网格蛋白介导的内吞作用所必需的。我们专注于 Src 家族激酶。我们发现Src 在 Tyr（490）处磷酸化 SYNJ2，从而刺激 SYNJ2 在体外的 5'-磷酸酶活性。我们还提供了证据表明 Src 介导的 SYNJ2 磷酸化有助于侵袭伪足的形成。
