Imperial College London, London, UK.
Cell Adh Migr. 2013 Jan-Feb;7(1):56-63. doi: 10.4161/cam.21831. Epub 2012 Oct 17.
The heterotrimeric laminins are a defining component of all basement membranes and self-assemble into a cell-associated network. The three short arms of the cross-shaped laminin molecule form the network nodes, with a strict requirement for one α, one β and one γ arm. The globular domain at the end of the long arm binds to cellular receptors, including integrins, α-dystroglycan, heparan sulfates and sulfated glycolipids. Collateral anchorage of the laminin network is provided by the proteoglycans perlecan and agrin. A second network is then formed by type IV collagen, which interacts with the laminin network through the heparan sulfate chains of perlecan and agrin and additional linkage by nidogen. This maturation of basement membranes becomes essential at later stages of embryo development.
异三聚体层粘连蛋白是所有基底膜的重要组成部分，并自行组装成细胞相关的网络。交叉形状的层粘连蛋白分子的三个短臂形成网络节点，严格要求一个α、一个β和一个γ臂。长臂末端的球形结构域与细胞受体结合，包括整合素、α- dystroglycan、硫酸乙酰肝素和硫酸糖脂。层粘连蛋白网络的副锚定由蛋白聚糖 perlecan 和 agrin 提供。然后，由 IV 型胶原形成第二个网络，它通过 perlecan 和 agrin 的硫酸乙酰肝素链与层粘连蛋白网络相互作用，并通过巢蛋白进行额外的连接。这种基底膜的成熟在胚胎发育的后期阶段变得至关重要。
