Leeds Institute of Molecular Medicine, University of Leeds, Leeds, UK.
J Clin Pathol. 2013 Mar;66(3):253-5. doi: 10.1136/jclinpath-2012-201147. Epub 2012 Oct 16.
Models considering breast cancer complexity cannot be easily or accurately replicated in routine cell line or animal models. We aimed to evaluate the practicality of organotypic tissue slice culture in breast cancer. Following ethical approval, 250 µm thick sections from surplus breast tumours (n=10) were prepared using a vibrating blade microtome. Triplicate tissue slices were placed in 6-well plates and cultured for up to 7 days ± tamoxifen (1 nM) or doxorubicin (1 µM). Tissue slices were fixed and embedded before sectioning for morphological evaluation and immunohistochemistry. H&E showed good preservation of tissue morphology. Collagen production was evident. Biomarkers of proliferation and apoptosis could be evaluated using immunohistochemistry and used as surrogates to quantify drug effects. In summary, breast cancer tissue slices can be cultured in vitro as organotypic models. Nevertheless, although simple in concept, the delicacy of the model with regard to handling makes subsequent analytical processes challenging.
考虑到乳腺癌的复杂性，模型不能轻易或准确地在常规细胞系或动物模型中复制。我们旨在评估乳腺癌器官型组织切片培养的实用性。在获得伦理批准后，使用振动刀片切片机从多余的乳腺癌肿瘤（n=10）中制备了 250µm 厚的切片。将 3 个组织切片放置在 6 孔板中，培养时间长达 7 天±他莫昔芬（1nM）或多柔比星（1µM）。组织切片在固定和包埋之前进行切片，用于形态学评估和免疫组织化学分析。H&E 显示组织形态保存良好。胶原生成明显。可以使用免疫组织化学评估增殖和凋亡的生物标志物，并用作定量药物作用的替代物。总之，乳腺癌组织切片可以作为器官型模型在体外培养。然而，尽管概念简单，但该模型在处理方面的精细程度使得后续的分析过程具有挑战性。
