Taliouri Eirini, Vrekoussis Thomas, Vergetaki Aikaterini, Agorastos Theodore, Makrigiannakis Antonis
Laboratory of Human Reproduction, Department of Obstetrics and Gynecology, Medical School, University of Crete, Heraklion, 710 03, Crete, Greece.
Tumour Biol. 2013 Feb;34(1):125-30. doi: 10.1007/s13277-012-0519-8. Epub 2012 Oct 18.
Corticotropin-releasing hormone acts as a stressor mediator in the human reproductive system. Corticotropin-releasing hormone (CRH) has been detected in several carcinomas of gynecological origin like breast, ovarian, and endometrial carcinomas. It was additionally shown that CRH could induce Fas ligand (FasL) expression in ovarian carcinoma cell lines. To determine whether CRH could also be expressed during cervical cancer development, we studied the expression of CRH using HeLa cells in an in vitro cervical cancer model. We further studied whether CRH could regulate FasL expression. In that context, HeLa cells were cultured in the presence or absence of 1 μM CRH. CRH and FasL expressions were assessed by indirect immunofluorescence, reverse transcription PCR, and Western blot. The current results indicated that in HeLa cells, CRH can significantly induce both FasL transcription and FasL translation. Taking into account previous studies already establishing a connection between FasL expression and tumor immunoescape in cervical cancer, it can be concluded that such immunoescape could be CRH dependent.
促肾上腺皮质激素释放激素在人类生殖系统中充当应激介质。在几种妇科来源的癌症如乳腺癌、卵巢癌和子宫内膜癌中已检测到促肾上腺皮质激素释放激素（CRH）。另外还表明，CRH可诱导卵巢癌细胞系中Fas配体（FasL）的表达。为了确定CRH在宫颈癌发生过程中是否也会表达，我们在体外宫颈癌模型中使用HeLa细胞研究了CRH的表达。我们进一步研究了CRH是否能调节FasL的表达。在此背景下，将HeLa细胞在存在或不存在1μM CRH的情况下进行培养。通过间接免疫荧光、逆转录PCR和蛋白质免疫印迹法评估CRH和FasL的表达。目前的结果表明，在HeLa细胞中，CRH可显著诱导FasL的转录和翻译。考虑到先前的研究已经在宫颈癌中建立了FasL表达与肿瘤免疫逃逸之间的联系，可以得出结论，这种免疫逃逸可能依赖于CRH。
