Department of Orthopaedics, University at Buffalo, Buffalo, New York 14226, USA.
Arthroscopy. 2012 Dec;28(12):1862-6. doi: 10.1016/j.arthro.2012.05.888. Epub 2012 Oct 16.
If an anterior cruciate ligament graft somehow becomes contaminated intraoperatively, soaking it in 4% chlorhexidine gluconate has been shown to be the most popular and efficacious method for sterilization before implantation. The purpose of this study was to evaluate the effects of a chlorhexidine soak on the structural properties of human patellar tendon allografts.
Sixteen human patellar tendon allografts were randomly split into 2 groups of 8. Grafts in 1 group were soaked in 4% chlorhexidine gluconate for 30 minutes, and the other grafts were kept moist in normal saline-soaked gauze. Data on preload width, preload thickness, elongation, ultimate tensile load, and stiffness were obtained through measurement and mechanical testing of the grafts.
Graft donor ages ranged from 29 to 43 years. There was no difference in the mean values of graft dimensions of the chlorhexidine-exposed group versus the normal saline-exposed group before mechanical testing (width of 9.48 mm v 9.56 mm, P = .89; thickness of 4.01 mm v 4.57 mm, P = .34). Graft elongation was not statistically different between the groups (2.52 mm v 1.43 mm, P = .27). No statistically significant difference was noted between the ultimate tensile load (2,219 N v 1,878 N, P = .36) or stiffness (274.3 N/mm v 297.0 N/mm, P = .63) of the grafts in both groups.
Structural properties of human patellar tendon allografts are not significantly affected by soaking in 4% chlorhexidine gluconate for 30 minutes.
Surgeons wishing to treat an inadvertently contaminated graft intraoperatively with 4% chlorhexidine may do so without concern that such treatment will impact graft strength.
如果前交叉韧带移植物在术中受到某种污染，将其浸泡在 4%葡萄糖酸氯己定中已被证明是植入前最受欢迎和最有效的灭菌方法。本研究旨在评估氯己定浸泡对人髌腱同种异体移植物结构性能的影响。
将 16 个人髌腱同种异体移植物随机分为 2 组，每组 8 个。一组移植物浸泡在 4%葡萄糖酸氯己定中 30 分钟，另一组移植物用生理盐水浸泡的纱布保持湿润。通过对移植物进行测量和机械测试，获得预加载宽度、预加载厚度、伸长率、极限拉伸载荷和刚度的数据。
移植物供体年龄为 29 至 43 岁。在机械测试前，氯己定暴露组与生理盐水暴露组的移植物尺寸平均值没有差异（宽度 9.48 毫米比 9.56 毫米，P=.89；厚度 4.01 毫米比 4.57 毫米，P=.34）。两组间移植物伸长率无统计学差异（2.52 毫米比 1.43 毫米，P=.27）。两组移植物的极限拉伸载荷（2219 牛比 1878 牛，P=.36）或刚度（274.3 牛/毫米比 297.0 牛/毫米，P=.63）均无统计学差异。
将人髌腱同种异体移植物浸泡在 4%葡萄糖酸氯己定中 30 分钟不会显著影响其结构性能。
希望在术中用 4%葡萄糖酸氯己定处理意外污染的移植物的外科医生可以这样做，而不必担心这种处理会影响移植物的强度。
