LEQUIA, Institute of the Environment, University of Girona, Campus Montilivi, E-17071 Girona, Catalonia, Spain.
Bioresour Technol. 2012 Dec;126:283-9. doi: 10.1016/j.biortech.2012.09.011. Epub 2012 Sep 13.
This study investigates the effects of temperature on ammonia oxidizing bacteria activity in a partial nitritation (PN) sequencing batch reactor. Stable PN was achieved in a 250 L SBR with a minimum operating volume of 111L treating mature landfill leachate containing an ammonium concentration of around 6000 mg N-NH(4)(+)L(-1) at both 25 and 35 °C. A suitable influent to feed an anammox reactor was achieved in both cases. A kinetic model was applied to study the influence of free ammonia (FA), the free nitrous acid (FNA) inhibition, and the inorganic carbon (IC) limitation. NH(4)(+) and NO(2)(-) concentrations were similar at 25 and 35 °C experiments (about 2500 mg N-NH(4)(+)L(-1) and 3500 mg N-NO(2)(-)L(-1)), FA and FNA concentrations differed due to the strong temperature dependence. FNA was the main source of inhibition at 25 °C, while at 35 °C combined FA and FNA inhibition occurred. DGGE results demonstrated that PN-SBR sludge was enriched on the same AOB phylotypes in both experiments.
本研究考察了温度对部分亚硝化（PN）序批式反应器中氨氧化菌活性的影响。在 250L 的 SBR 中实现了稳定的 PN，最小操作体积为 111L，处理含有约 6000mg N-NH（4）（+）L（-1）氨氮浓度的成熟垃圾渗滤液，在 25 和 35°C 下均可达到。在这两种情况下，均实现了适合于厌氧氨氧化反应器进料的进水。应用动力学模型研究了游离氨（FA）、游离亚硝酸（FNA）抑制和无机碳（IC）限制的影响。在 25 和 35°C 实验中，NH（4）（+）和 NO（2）（-）浓度相似（约 2500mg N-NH（4）（+）L（-1）和 3500mg N-NO（2）（-）L（-1）），FA 和 FNA 浓度因强烈的温度依赖性而不同。在 25°C 时，FNA 是主要的抑制源，而在 35°C 时，FA 和 FNA 联合抑制发生。DGGE 结果表明，PN-SBR 污泥在两种实验中均富集了相同的 AOB 类群。
