Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India.
Comp Immunol Microbiol Infect Dis. 2013 Jan;36(1):25-38. doi: 10.1016/j.cimid.2012.09.004. Epub 2012 Oct 15.
Wolbachia, the intracellular alpha-proteobacteria are required for the development, fertility and survival of filarial parasites. Wolbachia Translation initiation factor-1 (Wol Tl IF-1) is one of the factors required for Wolbachia growth and viability. In the present study, we cloned, over expressed and purified Wol Tl IF-1 that exhibited strong immuno-reactivity with various categories of bancroftian sera. Immunization with the recombinant protein resulted into significant reduction in microfilarial density (70-72%) and adult worm establishment (61-63%) in susceptible Mastomys coucha. Protection offered by Wol Tl IF-1 was found associated with humoral immune arm as observed by an increased antibody level with preponderance of IgE, IgM, IgG1 and IgG2a isotypes. The anti-Wol Tl IF-1 antibodies promoted profound adherence of peritoneal exudates cells to the surface of microfilariae and infective larvae causing cytotoxicity and their death. The present study indicates potential of recombinant Wol Tl IF-1 as a promising vaccine candidate against human lymphatic filarial infection.
沃尔巴克氏体（Wolbachia）是一种胞内的α-变形菌，是丝虫寄生虫发育、生育和存活所必需的。沃尔巴克氏体翻译起始因子-1（Wol Tl IF-1）是沃尔巴克氏体生长和存活所需的因素之一。在本研究中，我们克隆、过表达和纯化了 Wol Tl IF-1，它与各种类别的班氏丝虫血清表现出强烈的免疫反应性。用重组蛋白免疫可显著降低易感 Mastomys coucha 的微丝蚴密度（70-72%）和成虫建立（61-63%）。Wol Tl IF-1 提供的保护与体液免疫臂有关，如观察到抗体水平增加，以 IgE、IgM、IgG1 和 IgG2a 同种型为主。抗-Wol Tl IF-1 抗体促进了腹腔渗出细胞对微丝蚴和感染性幼虫表面的强烈粘附，导致细胞毒性和死亡。本研究表明重组 Wol Tl IF-1 作为一种有前途的抗人类淋巴丝虫感染疫苗候选物的潜力。
