巴西甘薯轻斑驳病毒的全基因组序列。
The complete genome sequence of a Brazilian isolate of yam mild mosaic virus.
机构信息
Laboratório de Fitovirologia, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, CEP 52171-900, Recife, PE, Brazil.
出版信息
Arch Virol. 2013 Feb;158(2):515-8. doi: 10.1007/s00705-012-1509-2. Epub 2012 Oct 19.
In this study, the complete genome of an isolate of yam mild mosaic virus (YMMV) from Brazil was sequenced, and the predicted amino acid sequence was analyzed. The YMMV RNA genome consists of 9538 nt without the poly(A) tail, encoding a putative typical potyvirus polyprotein of 3084 amino acids. Furthermore, the small overlapping ORF (PIPO) in the P3 gene was also deduced, and the cleavage sites of the polyprotein were predicted. Multiple alignment with other potyviruses showed a maximum nucleotide sequence identity of 64 % to wild tomato mosaic virus. A phylogenetic tree showed that YMMV clustered with Asian potyviruses that mainly infect solanaceous plants.
在这项研究中，对来自巴西的山药轻斑驳病毒（YMMV）分离株的完整基因组进行了测序，并对预测的氨基酸序列进行了分析。YMMV RNA 基因组由 9538 个核苷酸组成，没有 poly(A)尾巴，编码一个假定的典型马铃薯 Y 病毒多聚蛋白，由 3084 个氨基酸组成。此外，还推导出了 P3 基因中的小重叠 ORF（PIPO），并预测了多聚蛋白的切割位点。与其他马铃薯 Y 病毒的多重比对显示，与野生番茄花叶病毒的核苷酸序列同一性最高为 64%。系统发育树表明，YMMV 与主要感染茄科植物的亚洲马铃薯 Y 病毒聚类在一起。
Zotero 插件