Division of Reproductive Medicine, Department of Obstetrics and Gynecology, University Medical Centre Ljubljana, Ljubljana, Slovenia.
PLoS One. 2012;7(10):e47106. doi: 10.1371/journal.pone.0047106. Epub 2012 Oct 17.
In in vitro fertilization (IVF) cycles controlled ovarian hyperstimulation (COH) is established by gonadotropins in combination with gonadotropin-releasing hormone (GnRH) agonists or antagonists, to prevent premature luteinizing hormone (LH) surge. The aim of our study was to improve the understanding of gene expression profile of cumulus cells (CC) in terms of ovarian stimulation protocol and oocyte maturity. We applied Affymetrix gene expression profiling in CC of oocytes at different maturation stages using either GnRH agonists or GnRH antagonists. Two analyses were performed: the first involved CC of immature metaphase I (MI) and mature metaphase II (MII) oocytes where 359 genes were differentially expressed, and the second involved the two GnRH analogues where no differentially expressed genes were observed at the entire transcriptome level. A further analysis of 359 differentially genes was performed, focusing on anti-Müllerian hormone receptor 2 (AMHR2), follicle stimulating hormone receptor (FSHR), vascular endothelial growth factor C (VEGFC) and serine protease inhibitor E2 (SERPINE2). Among other differentially expressed genes we observed a marked number of new genes connected to cell adhesion and neurotransmitters such as dopamine, glycine and γ-Aminobutyric acid (GABA). No differential expression in CC between the two GnRH analogues supports the findings of clinical studies where no significant difference in live birth rates between both GnRH analogues has been proven.
在体外受精（IVF）周期中，通过促性腺激素与促性腺激素释放激素（GnRH）激动剂或拮抗剂联合使用来建立控制性卵巢过度刺激（COH），以防止过早的黄体生成素（LH）激增。我们的研究旨在通过卵巢刺激方案和卵母细胞成熟来更好地理解卵丘细胞（CC）的基因表达谱。我们使用 GnRH 激动剂或 GnRH 拮抗剂，对不同成熟阶段的卵母细胞中的 CC 进行了 Affymetrix 基因表达谱分析。进行了两次分析：第一次涉及不成熟中期 I（MI）和成熟中期 II（MII）卵母细胞的 CC，其中有 359 个基因表达差异，第二次涉及两种 GnRH 类似物，在整个转录组水平上未观察到差异表达基因。对 359 个差异表达基因进行了进一步分析，重点关注抗苗勒氏管激素受体 2（AMHR2）、卵泡刺激素受体（FSHR）、血管内皮生长因子 C（VEGFC）和丝氨酸蛋白酶抑制剂 E2（SERPINE2）。在其他差异表达基因中，我们观察到大量与细胞黏附以及多巴胺、甘氨酸和γ-氨基丁酸（GABA）等神经递质相关的新基因。两种 GnRH 类似物之间 CC 没有差异表达，这支持了临床研究的发现，即两种 GnRH 类似物之间的活产率没有显著差异。
