采用毛细管气相色谱法测定人卵巢卵泡液中的类固醇。

Steroid determinations in human ovarian follicular fluid using capillary gas chromatography.

作者信息

Vanluchene E, Hinting A, Dhont M, Serreyn R, Vandekerckhove D

机构信息

Department of Gynecology, State University of Ghent, Belgium.

出版信息

J Steroid Biochem. 1990 Jan;35(1):83-9. doi: 10.1016/0022-4731(90)90149-m.

DOI:10.1016/0022-4731(90)90149-m

PMID:2308331

Abstract

A method is presented based on capillary GLC using both a thermionic and a flame ionization detector to simultaneously analyse all major unconjugated steroids in ovarian follicular fluids (FF). Although specificity can not always be guaranteed for the smaller concentrations of androstenedione and cortisol, accuracy and reproducibility are excellent for the major progestagens and estrogens (progesterone, 17- and 16 alpha-hydroxyprogesterone, pregnenolone, 20 alpha-dihydroprogesterone, estradiol and estrone). Above all the analysis is performed with relatively cheap instrumentation and products. Apart from the "profiles" of unconjugated steroids, a semi-quantitative analysis of steroid conjugates is possible if a preliminary group separation with disposable anion exchanger columns is included.

摘要

本文介绍了一种基于毛细管气相色谱法的方法，该方法使用热离子检测器和火焰离子化检测器同时分析卵巢卵泡液（FF）中的所有主要游离甾体。尽管对于较小浓度的雄烯二酮和皮质醇，特异性并非总能得到保证，但对于主要的孕激素和雌激素（孕酮、17α-和16α-羟基孕酮、孕烯醇酮、20α-二氢孕酮、雌二醇和雌酮），准确性和重现性极佳。最重要的是，该分析使用相对便宜的仪器和产品进行。除了游离甾体的“图谱”外，如果包括使用一次性阴离子交换柱进行初步分组分离，还可以对甾体结合物进行半定量分析。