Department of Microbiology, Østfold Hospital Trust, Fredrikstad, Norway.
APMIS. 2013 Apr;121(4):353-8. doi: 10.1111/apm.12009. Epub 2012 Oct 22.
The aim of the study was to describe the antimicrobial resistance rate of Helicobacter pylori isolated from previously untreated patients in Norway, including the application of two different methods for the determination of metronidazole susceptibility. Altogether 102 isolates obtained in 2008 and 2009 from previously untreated patients suspected of H. pylori related disease, were examined applying a standardized European study protocol. The activity of amoxicillin, tetracycline, clarithromycin, metronidazole, rifabutin and levofloxacin was recorded after an incubation period of 72-96 h in a microaerobic atmosphere. Strains resistant to metronidazole were re-examined for metronidazole resistance applying anaerobic conditions for the first 24 h. None of the isolates were resistant to amoxicillin or tetracycline, whereas 5, 9% were resistant to clarithromycin and 22, 5% resistant to metronidazole tested conventionally. Applying local standards the metronidazole resistance rate fell to 7, 8%, highlighting the importance of the methodology applied for metronidazole susceptibility testing.
本研究旨在描述 2008 年至 2009 年间，挪威未经治疗的幽门螺杆菌分离株的抗生素耐药率，包括两种不同的甲硝唑药敏检测方法的应用。根据标准化的欧洲研究方案，对怀疑与幽门螺杆菌相关疾病的 102 例未经治疗的患者中分离出的幽门螺杆菌进行检测。在微需氧条件下孵育 72-96 小时后，记录阿莫西林、四环素、克拉霉素、甲硝唑、利福布汀和左氧氟沙星的活性。对甲硝唑耐药的菌株，先在厌氧条件下培养 24 小时，然后重新检测甲硝唑耐药性。没有分离株对阿莫西林或四环素耐药，而 5.9%的分离株对克拉霉素耐药，22.5%的分离株对常规检测的甲硝唑耐药。采用当地标准，甲硝唑耐药率下降至 7.8%，突出了甲硝唑药敏检测方法的重要性。
