Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhongshan 2nd Road, Guangzhou 510080, Guangdong Province, China.
Biochem Biophys Res Commun. 2012 Nov 16;428(2):309-14. doi: 10.1016/j.bbrc.2012.10.053. Epub 2012 Oct 22.
Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.
基质金属蛋白酶-1（MMP-1）是锌依赖性内肽酶家族的一员，能够降解细胞外基质（ECM）和某些非基质蛋白。已经表明，MMP-1 可以通过改善成肌细胞的分化和迁移来增强肌肉再生。然而，目前尚不清楚 MMP-1 是否可以促进骨髓间充质干细胞（BMSCs）的成肌作用。为了解决这个问题，我们从 C57BL/6J 小鼠中分离出 BMSCs，并研究了 MMP-1 对其增殖和成肌分化的影响。我们的结果表明，MMP-1 处理对 BMSCs 没有细胞毒性作用，而是以剂量依赖性方式增加了 MyoD 和结蛋白的 mRNA 和蛋白水平，表明 MMP-1 促进了 BMSCs 的体外成肌分化。这些结果表明，BMSCs 可能具有治疗肌肉疾病的治疗潜力。
