Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
World J Microbiol Biotechnol. 2013 Mar;29(3):441-6. doi: 10.1007/s11274-012-1196-x. Epub 2012 Oct 21.
(+)-Terrein is a fungal metabolite with multiple biological activities, especially with great value in medicine. However, the mass production of single configuration terrein is still a big challenge. In this study, the effects of acetic acid, sodium acetate, citric acid and sodium citrate on the (+)-terrein production by Aspergillus terreus strain PF26 derived from marine sponge Phakellia fusca were investigated. Sodium citrate was selected for fed-batch cultivation because it showed the best effect on (+)-terrein production among the four regulators tested. As a result, 5.38 g/L (+)-terrein production was achieved by feeding 10 mM sodium citrate on the 3rd day in shake flask, which was 33.8 % higher than the control and represented the highest yield of (+)-terrein. In a 7.5-L stirred bioreactor, 2.58 g/L of (+)-terrein production was achieved by the feeding of 10 mM sodium citrate on the 8th day. The results from this study lay a basis for the high-yield production of (+)-terrein by fermentation.
(+)-Terrein 是一种具有多种生物活性的真菌代谢产物，特别是在医学上具有很大的价值。然而，单一构型的(+)-terrein 的大规模生产仍然是一个巨大的挑战。在本研究中，考察了乙酸、醋酸钠、柠檬酸和柠檬酸钠对海洋海绵 Phakellia fusca 来源的曲霉菌株 PF26 生产(+)-terrein 的影响。由于在四种调节剂中，柠檬酸钠对(+)-terrein 生产的效果最好，因此选择其进行补料分批培养。结果，在摇瓶中，第三天补加 10 mM 柠檬酸钠可实现 5.38 g/L 的 (+)-terrein 生产，比对照提高了 33.8%，达到了(+)-terrein 的最高产量。在 7.5 L 搅拌式生物反应器中，第八天补加 10 mM 柠檬酸钠可实现 2.58 g/L 的 (+)-terrein 生产。本研究结果为发酵法高产 (+)-terrein 奠定了基础。
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