用柠檬酸钠增强土曲霉 PF26 分批补料培养生产（+）-土霉素。

Enhanced production of (+)-terrein in fed-batch cultivation of Aspergillus terreus strain PF26 with sodium citrate.

机构信息

Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

出版信息

World J Microbiol Biotechnol. 2013 Mar;29(3):441-6. doi: 10.1007/s11274-012-1196-x. Epub 2012 Oct 21.

DOI:10.1007/s11274-012-1196-x

PMID:23085955

Abstract

(+)-Terrein is a fungal metabolite with multiple biological activities, especially with great value in medicine. However, the mass production of single configuration terrein is still a big challenge. In this study, the effects of acetic acid, sodium acetate, citric acid and sodium citrate on the (+)-terrein production by Aspergillus terreus strain PF26 derived from marine sponge Phakellia fusca were investigated. Sodium citrate was selected for fed-batch cultivation because it showed the best effect on (+)-terrein production among the four regulators tested. As a result, 5.38 g/L (+)-terrein production was achieved by feeding 10 mM sodium citrate on the 3rd day in shake flask, which was 33.8 % higher than the control and represented the highest yield of (+)-terrein. In a 7.5-L stirred bioreactor, 2.58 g/L of (+)-terrein production was achieved by the feeding of 10 mM sodium citrate on the 8th day. The results from this study lay a basis for the high-yield production of (+)-terrein by fermentation.

摘要

(+)-Terrein 是一种具有多种生物活性的真菌代谢产物，特别是在医学上具有很大的价值。然而，单一构型的(+)-terrein 的大规模生产仍然是一个巨大的挑战。在本研究中，考察了乙酸、醋酸钠、柠檬酸和柠檬酸钠对海洋海绵 Phakellia fusca 来源的曲霉菌株 PF26 生产(+)-terrein 的影响。由于在四种调节剂中，柠檬酸钠对(+)-terrein 生产的效果最好，因此选择其进行补料分批培养。结果，在摇瓶中，第三天补加 10 mM 柠檬酸钠可实现 5.38 g/L 的 (+)-terrein 生产，比对照提高了 33.8%，达到了(+)-terrein 的最高产量。在 7.5 L 搅拌式生物反应器中，第八天补加 10 mM 柠檬酸钠可实现 2.58 g/L 的 (+)-terrein 生产。本研究结果为发酵法高产 (+)-terrein 奠定了基础。