Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany.
Biol Chem. 2013 Feb;394(2):261-70. doi: 10.1515/hsz-2012-0282.
Adenosine 3',5'-cyclic monophosphate and guanosine 3',5'-cyclic monophosphate are second messengers that regulate multiple physiological functions. The existence of additional cyclic nucleotides in mammalian cells was postulated many years ago, but technical problems hampered development of the field. Using highly specific and sensitive mass spectrometry methods, soluble guanylyl cyclase has recently been shown to catalyze the formation of several cyclic nucleotides in vitro. This minireview discusses the broad substrate-specificity of soluble guanylyl cyclase and the possible second messenger roles of cyclic nucleotides other than adenosine 3',5'-cyclic monophosphate and guanosine 3',5'-cyclic monophosphate. We hope that this article stimulates productive and critical research in an area that has been neglected for many years.
腺苷酸 3'，5'-环单磷酸和鸟苷酸 3'，5'-环单磷酸是调节多种生理功能的第二信使。多年前就有人假设哺乳动物细胞中存在其他环状核苷酸，但技术问题阻碍了该领域的发展。最近，使用高度特异性和灵敏的质谱方法，已证明可溶性鸟苷酸环化酶可在体外催化几种环状核苷酸的形成。这篇综述讨论了可溶性鸟苷酸环化酶广泛的底物特异性，以及除腺苷酸 3'，5'-环单磷酸和鸟苷酸 3'，5'-环单磷酸之外的环状核苷酸可能作为第二信使的作用。我们希望本文能激发多年来被忽视的这一领域富有成效和批判性的研究。
