Dental Hygienist Education, Department of Odontology, Dental School, Umeå University, Umeå, Sweden.
Acta Odontol Scand. 2013 May-Jul;71(3-4):883-91. doi: 10.3109/00016357.2012.734414. Epub 2012 Oct 22.
The aim of this study was to evaluate the effect on mutans streptococci and lactobacilli in saliva of mouthrinsing with essential oils and an alcohol-free chlorhexidine.
Twenty healthy volunteers (mean age 59 years) participated in the double-blind randomized cross-over study. Three mouthrinses were used in 16 days rinsing periods in addition to their regular mechanical oral hygiene: a solution with essential oils (EO; Listerine), a solution with alcohol-free chlorhexidine (CHX; Paroex) and water (negative control). The mouthrinse periods were separated by 3-month washout periods. At days 0 (baseline) and 17 (end) of each mouthrinse period, paraffin stimulated whole saliva was collected in order to analyse CFU/ml saliva of mutans streptococci and lactobacilli.
Only the CHX rinse showed a significant difference for CFU mutans streptococci between baseline and end (p = 0.004). The CFU mutans streptococci at the end of the rinse periods showed statistically significant differences between CHX vs EO (p = 0.039) and CHX vs water (p = 0.022). The difference in CFU lactobacilli between baseline and end was significant for CHX (p = 0.031), but not for the other rinses. No statistically significant differences for lactobacilli were found at the end of the rinse periods between the mouthrinses.
A significant reduction in amount of cariogenic bacteria in saliva was observed after 16 days of alcohol-free chlorhexidine mouthrinse but not after the essential oils rinse. The high number of participant's not changing to a bacterial class with a reduced number of micro-organisms showed that both rinses had little clinical significance as a caries preventing treatment method, which can decrease the number of CFU cariogenic micro-organisms.
本研究旨在评估用精油和无酒精洗必泰漱口对唾液中变形链球菌和乳杆菌的影响。
20 名健康志愿者（平均年龄 59 岁）参加了这项双盲随机交叉研究。在 16 天的漱口期间，除了常规的机械口腔卫生外，他们还使用了 3 种漱口液：含精油（Listerine）的溶液、含无酒精洗必泰（Paroex）的溶液和水（阴性对照）。漱口期之间间隔 3 个月的洗脱期。在每个漱口期的第 0 天（基线）和第 17 天（结束），收集石蜡刺激全唾液，以分析唾液 CFU/ml 中的变形链球菌和乳杆菌。
只有洗必泰漱口液在基线和结束时 CFU 变形链球菌之间显示出显著差异（p = 0.004）。漱口期结束时 CFU 变形链球菌在洗必泰和精油之间（p = 0.039）和洗必泰和水之间（p = 0.022）存在统计学显著差异。CHX 组在基线和结束时 CFU 乳杆菌的差异有统计学意义（p = 0.031），但其他漱口液则无。漱口期结束时，各组之间 CFU 乳杆菌无统计学差异。
经过 16 天的无酒精洗必泰漱口后，唾液中致龋菌数量显著减少，但用精油漱口后则没有。由于大量参与者没有转变为细菌数量减少的细菌种类，这表明两种漱口液作为一种防龋治疗方法，临床意义不大，因为它们可以减少 CFU 致龋微生物的数量。
