基孔肯雅病毒3'非翻译区中三个重复序列元件的确认。

The confirmation of three repeated sequence elements in the 3' untranslated region of Chikungunya virus.

作者信息

Zhang Yu, Si Bing-Yin, Kang Xiao-Ping, Hu Yi, Li Jing, Wu Xiao-Yan, Li Yu-Chang, Yang Yin-Hui, Zhu Qing-Yu

机构信息

State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dongda Street, Fengtai District, Beijing, China.

出版信息

Virus Genes. 2013 Feb;46(1):165-6. doi: 10.1007/s11262-012-0835-5. Epub 2012 Oct 23.

DOI:10.1007/s11262-012-0835-5

PMID:23090832

Abstract

In this study, the complete genomic nucleotide sequence of Chikungunya virus (CHIKV) strain S27 African prototype was determined and three 21 nucleotides repeated sequence elements (RSEs) at positions 11398-11418, 11533-11553, and 11620-11640 in the 3' untranslated region (3'UTR) were confirmed. In addition, the 3'UTRs of all CHIKV strains deposited in GenBank were analyzed. The results displayed that the majority of the CHIKV strains consisted of the three 21 nucleotides RSEs in the 3'UTRs, and the third RSE was the most conservative. The conservation of the three RSEs of 21 nucleotides within the 3'UTR of CHIKV genome may play an important role on the virus replication cycle.

摘要

在本研究中，测定了基孔肯雅病毒（CHIKV）S27非洲原型株的完整基因组核苷酸序列，并确认了3'非翻译区（3'UTR）中位置11398 - 11418、11533 - 11553和11620 - 11640处的三个21核苷酸重复序列元件（RSEs）。此外，对GenBank中保存的所有CHIKV株的3'UTR进行了分析。结果显示，大多数CHIKV株在3'UTR中包含这三个21核苷酸RSEs，且第三个RSE最为保守。CHIKV基因组3'UTR内21核苷酸的三个RSEs的保守性可能在病毒复制周期中起重要作用。