Pfeffer M, Kinney R M, Kaaden O R
Institute for Medical Microbiology, Epidemic and Infectious Diseases, Veterinary Faculty, Ludwig-Maximilians University, Munich, Germany.
Virology. 1998 Jan 5;240(1):100-8. doi: 10.1006/viro.1997.8907.
The 3'-nontranslated region (NTR) of representative strains of all known alphavirus species was amplified by reverse transcription-polymerase chain reaction. For 23 of them, the 3'-NTR sequence was determined. Together with previously published data, this allowed an analysis of the 3'-NTR of the viruses in the genus Alphavirus. The length of the 3'-NTRs varied from 77 nt for Pixuna virus to 609 nt for Bebaru virus. The 19-nt conserved sequence element directly adjacent to the poly(A) tract was found in all viruses, supporting the hypothesis that this region is a cis-acting sequence element during viral replication and essential for virus growth in vitro. Within the 3'-NTR of all alphaviruses, repeated sequence elements of various numbers and lengths were found. Their composition was very consistent in both the Venezuelan equine encephalitis (VEE) and the Sindbis-like viruses, although their number was constant only within the latter group. For the VEE viruses, our data suggested that insertion events rather than deletions from an ancestor with a long 3'-NTR created the various number of repeated sequence elements. Among the remaining viruses, both the number and the composition of repeated sequence elements varied remarkedly.
通过逆转录-聚合酶链反应扩增了所有已知甲病毒属代表毒株的3'-非翻译区（NTR）。其中23株病毒的3'-NTR序列得到了测定。结合此前发表的数据，得以对甲病毒属病毒的3'-NTR进行分析。3'-NTR的长度从皮克苏纳病毒的77个核苷酸到贝巴鲁病毒的609个核苷酸不等。在所有病毒中均发现了紧邻多聚（A）尾的19个核苷酸的保守序列元件，这支持了该区域在病毒复制过程中是顺式作用序列元件且对病毒体外生长至关重要的假说。在所有甲病毒的3'-NTR内，发现了数量和长度各异的重复序列元件。委内瑞拉马脑炎（VEE）病毒和辛德比斯样病毒中它们的组成非常一致，不过只有在后者组内其数量是恒定的。对于VEE病毒，我们的数据表明，从具有长3'-NTR的祖先发生的插入事件而非缺失事件产生了不同数量的重复序列元件。在其余病毒中，重复序列元件的数量和组成均有显著差异。
