铅对体内和体外红细胞胆色素原脱氨酶活性的影响。

Effect of lead on the activity of erythrocyte porphobilinogen deaminase in vivo and in vitro.

作者信息

Tomokuni K, Ichiba M

机构信息

Department of Community Health Science, Saga Medical School, Japan.

出版信息

Toxicol Lett. 1990 Feb;50(2-3):137-42. doi: 10.1016/0378-4274(90)90003-5.

DOI:10.1016/0378-4274(90)90003-5

PMID:2309232

Abstract

The effect of lead on the activity of erythrocyte porphobilinogen deaminase (PBGD) in vivo and in vitro was investigated using blood specimens obtained from controls and lead-exposed workers. When lead nitrate was added to the incubation mixture at a final concentration of 10(-4) M, 83% inhibition of erythrocyte PBGD activity was found. However, in workers occupationally exposed to lead, no inhibition of erythrocyte PBGD activity was detected. This finding indicates that the erythrocyte PBGD test is not useful for evaluating exposure to lead in workers. In addition, the in vitro study confirmed that mercuric chloride strongly inhibits erythrocyte PBGD activity.

摘要

利用从对照组和铅暴露工人采集的血样，研究了铅在体内和体外对红细胞胆色素原脱氨酶（PBGD）活性的影响。当向孵育混合物中添加终浓度为10⁻⁴ M的硝酸铅时，发现红细胞PBGD活性受到83%的抑制。然而，在职业性铅暴露工人中，未检测到红细胞PBGD活性受到抑制。这一发现表明，红细胞PBGD检测对于评估工人的铅暴露情况并无用处。此外，体外研究证实，氯化汞可强烈抑制红细胞PBGD活性。