Guzmán Ana María, Sánchez Tomás, de la Barra Ricardo
Departamento de Laboratorios Clínicos, Pontificia Universidad Católica de Chile, Santiago, Chile.
Rev Chilena Infectol. 2012 Aug;29(4):406-11. doi: 10.4067/S0716-10182012000400007.
Blood culture is considered the "gold standard" for the diagnosis of bacteremia, critical condition with high morbidity and mortality. Because of its importance, it is estimated that the blood culture is a critical test that requires close monitoring on the quality with which the process is performed. The objective of this work is to show the results of the monitoring carried out during the past three years, of 5 quality indicators of blood cultures in the laboratory of the Hospital Clínico de la Pontificia Universidad Católica de Chile, considering pre-analytical, analytical and post-analytical aspects. In the 3 years monitored the mean contamination was 0,7%, 46% of adult bottles had adequate volume, match between Gram stain with final identification was 99.4%, 100% of correct participations were achieved in surveys of external quality control and Gram staining notification before 1 hour was 88.7%. With regard to proposed aims, in 2011 the laboratory complies with all, except the percentage of bottles with appropriate volume of blood inoculated. This indicator is very low and should be corrected as soon as possible since it is known that it is an important condition for optimum performance of blood cultures.
血培养被认为是诊断菌血症的“金标准”，菌血症是一种发病率和死亡率都很高的危急病症。鉴于其重要性，据估计血培养是一项关键检测，其操作过程的质量需要密切监测。本文的目的是展示过去三年在智利天主教大学临床医院实验室对血培养的5项质量指标进行监测的结果，这些指标涵盖分析前、分析中和分析后阶段。在监测的三年中，平均污染率为0.7%，46%的成人血培养瓶血量充足，革兰氏染色与最终鉴定的匹配率为99.4%，外部质量控制调查的正确参与率达到100%，1小时内革兰氏染色报告率为88.7%。关于既定目标，2011年该实验室除接种血液量合适的培养瓶百分比外，其他各项均达标。该指标非常低，应尽快纠正，因为众所周知这是血培养最佳效果的一个重要条件。
