Khaenam Prasong, Niibori Akiko, Okada Seiji, Jearanaikoon Patcharee, Araki Norie, Limpaiboon Temduang
Department of Biomedical Sciences, Graduate School, Khon Kaen University, Khon Kaen, Thailand.
Asian Pac J Cancer Prev. 2012;13(8):4007-11. doi: 10.7314/apjcp.2012.13.8.4007.
Retinoblastoma-interacting zinc finger gene (RIZ1) is a tumor suppressor gene which is highly inactivated by promoter hypermethylation in patients with liver fluke-related cholangiocarcinoma (CCA). Epigenetic aberration of this gene might withdraw the ability to restrain tumor cell proliferation and migration. We aimed to define the role of RIZ1 on cell proliferation and migration in CCA cell line.
Small interference RNA (siRNA) was used to knock down the expression of RIZ1 in a CCA-derived cell line in which cell proliferation and cell migration were performed.
A predominant nuclear localization of RIZ1 was observed. Reduction of RIZ1 by siRNA augmented cell proliferation and migration.
The result suggested that RIZ1 might play a role in regulating cell proliferation and migration in CCA. Reduction of RIZ1 expression may aggravate the progression of CCA.
视网膜母细胞瘤相互作用锌指基因（RIZ1）是一种肿瘤抑制基因，在肝吸虫相关胆管癌（CCA）患者中，该基因因启动子高甲基化而高度失活。该基因的表观遗传异常可能会丧失抑制肿瘤细胞增殖和迁移的能力。我们旨在确定RIZ1在CCA细胞系中对细胞增殖和迁移的作用。
使用小干扰RNA（siRNA）敲低源自CCA的细胞系中RIZ1的表达，并在此细胞系中进行细胞增殖和细胞迁移实验。
观察到RIZ1主要定位于细胞核。通过siRNA降低RIZ1水平可增强细胞增殖和迁移能力。
结果表明RIZ1可能在调节CCA细胞增殖和迁移中发挥作用。RIZ1表达降低可能会加剧CCA的进展。
